67ga citrate

For SPECT, 4.1–5.4 MBq of ⁶⁷Ga-citrate (CIS Bio, France) was injected iv. in 0.2 mL 24 h before imaging A 45-min SPECT scan was then performed using a NanoSPECT/CT Silver Upgrade small animal SPECT system (Mediso Ltd, Hungary) using both the 93 keV and 184 keV photopeaks in a 20–20% energy window, with 60 acquisition views and a Monte–Carlo model based three-dimensional SPECT reconstruction (Tera-Tomo, Mediso Ltd, Hungary) resulting in an image volume of 0.6 mm voxels. The same animal in the same animal bed allowing constant position was then immediately subjected to a whole-body three-dimensional MR imaging sequence in the nanoScan 1T MRI subcomponent. After these acquisitions, tissues were examined with ex vivo CLI, histology and immunohistochemistry following the in vivo imaging. All live animal image acquisitions were performed under 2% isoflurane anesthesia in medical oxygen in a special cross-compatible animal holder bed (MultiCell, Mediso, Hungary) which kept the animals immobilised during cross-modality and cross-device scans.