For freezing, Quantifoil 1.2/1.3 Cu 300 mesh grids (Quantifoil Micro Tools) were glow-discharged in methanol vapor before sample application. 3 μl sample was applied to each grid, which was then blotted for 1.5 s and plunge-frozen into liquid ethane using a FEI Vitrobot Mark IV. Micrographs were collected on an FEI Titan Krios 300 kV microscope with a post energy filter Gatan K2-Summit direct detector camera. Movies were collected at nominal 165,000× magnification, equivalent to a pixel spacing of 0.82 Å. A total dose of 40.8 e−/Å2 was used to collect 40 frames over 6 s, using a nominal defocus range covering −2.0 to −3.8 μm. This defocus range was selected to optimize signal-noise ratio/contrast, which was relatively low because of preferential distribution of particles in regions of very thick ice.
1.2 1 3 cu 300 mesh grids
Manufactured by Quantifoil
Quantifoil 1.2/1.3 Cu 300 mesh grids are a type of electron microscopy sample support. They feature a continuous carbon film with precisely arranged circular holes, supported on a copper mesh. The core function of these grids is to provide a stable and standardized substrate for preparing and analyzing samples using transmission electron microscopy (TEM).
Automatically generated - may contain errors
2 protocols using 1.2 1 3 cu 300 mesh grids
1
Cryo-EM Sample Preparation Protocol
2
Cryo-EM Sample Preparation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For freezing, Quantifoil 1.2/1.3 Cu 300 mesh grids (Quantifoil Micro Tools) were glow-discharged in methanol vapor prior to sample application. 3 μl sample was applied to each grid, which was then blotted for 1.5 s and plunge-frozen into liquid ethane using a FEI Vitrobot Mark IV. Micrographs were collected on an FEI Titan Krios 300 kV microscope with a post energy filter Gatan K2-Summit direct detector camera. Movies were collected at nominal 165,000x magnification, equivalent to a pixel spacing of 0.82Å. A total dose of 40.8 e-/Å 2 was used to collect 40 frames over 6 sec, using a nominal defocus range covering -2.0 to -3.8 µm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!