Dv 2 pro extra viscometer

The Brookfield DV-II + Pro EXTRA viscometer was used to measure solvent viscosity, and the G LAB melting point apparatus was used to measure the melting point of solvent and derivatives. The ¹H, and ¹³C NMR spectra, CHNS/O elemental analyzer, and mass analysis of compounds were prepared using the Varian Inova 500MHz, the EMA 502, and the Agilent technologies 5975C. The Thermo biomate 5 Spectrophotometer was used to prepare the suspension of bacteria. The suspension of bacteria was obtained from the American Type Culture Collection (ATCC). All the reagent materials used for the synthesis of deep eutectic solvent and the synthesis of pyrazole derivatives and pyrano[2,3-c] pyrazole derivatives such as choline chloride, urea, malononitrile, aldehyde derivatives, and hydrazine derivatives were obtained from Merck and Sigma.

The EA-0 and EA-POSS copolymers were synthesized via free radical batch copolymerization of A-POSS (0–0.5 mol), BA (7.5–8 moles), GMA (1 mol), HBA (1 mol) and ABP (0.001 mol) in ethyl acetate using AIBN as an initiator (0.1 wt part/100 wt parts of the monomers). The copolymerization process was realized at 78 °C for 5 h in a glass reactor equipped with a mechanical stirrer. The prepared products (i.e., copolymer solutions) contained 50 wt % of solids. Composition of the EA-0 and EA-POSS is tabulated in Table 1, while their theoretical structure is shown in Figure 2. A photograph of the copolymer solutions is presented in Figure 3.
Dynamic viscosity of the EA-0 and EA-POSS solutions was measured at 23 °C by means of the DV-II Pro Extra viscometer (spindle #7, 50 rpm; Brookfield, New York, NY, USA). Gel permeation chromatography (GPC-MALLS) was used for determination of molecular masses (Mw, Mn) and polydispersity (PDI) of the copolymers; the GPC apparatus contained the differential refractive index detector (Dn-2010 RI WGE Dr. Bures), the multiangle laser light scattering detector (DAWN EOS, Wyatt Technologies, Santa Barbara, CA, USA) and the columns: PSS 100 Å, PSS 500 Å, PSS 1000 Å and PSS 100000 Å (Polymer Standard Service, Mainz, Germany). The GPC tests were performed using THF and polystyrene standards.

The dynamic viscosity of the MAT solutions was determined at room temperature using the DV-II Pro Extra viscometer (spindle #7, 50 rpm; Brookfield, New York, NY, USA).