FTIR spectroscopic images were collected using a Nicolet iN10 MX infrared microscope (Thermo Fisher Scientific, Waltham, MA, USA) equipped with a liquid nitrogen cooled 16-element mercury-cadmium-telluride linear array detector. The OMNIC Picta software 9.0 (Thermo Fisher Scientific, Waltham, MA, USA) was used for instrument control and spectral data acquisition. In this study, the spatial and spectral resolutions were set to 25 × 25 μm2 and 4 cm−1. Spectra were acquired with the co-addition of 32 scans over the range 4000−900 cm−1. A background image was collected from a blank substrate before the collection of each sample image. FTIR spectroscopic images of samples mounted on CaF2 slides were collected in transmission mode. In these conditions, approximately 4 min were needed to collect an infrared image containing 160 spectra and corresponding to a typical hypothalamus area of 250 × 400 μm2. It should be mentioned that before each measurement, the specific area of hypothalamus was confirmed by a professional pathologist. A total of 47 images (an image per sample) were finally recorded. A row spectral data set containing 7520 spectra (2720 for the fatal hypothermia group, 2720 for the fatal hyperthermia group and 2080 for the normothermia group) were gathered from these 47 images and then subjected to data pre-processing.
Nicolet in10 mx infrared microscope
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Nicolet iN10 MX is an infrared microscope designed for analytical applications. It provides high-resolution imaging and spectroscopy capabilities for the examination of small samples. The instrument is equipped with a range of features to enable efficient data collection and analysis.
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Lab products found in correlation
Escalab 250, by Thermo Fisher Scientific (6 mentions)
Noran system six, by Thermo Fisher Scientific (1 mentions)
Empyrean powder diffractometer, by Malvern Panalytical (1 mentions)
Dpx 400, by Bruker (1 mentions)
7820 gc system, by Agilent Technologies (1 mentions)
Jsm 7400f, by JEOL (1 mentions)
Empyrean diffractometer, by Malvern Panalytical (1 mentions)
Omnicpicta software, by Thermo Fisher Scientific (1 mentions)
6 protocols using nicolet in10 mx infrared microscope
1
FTIR Imaging of Hypothalamus Thermoregulation
2
Comprehensive Characterization of Materials
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The Fourier-transform infrared spectroscopy (FTIR) spectra were collected from a Thermo Scientific Nicolet iN 10Mx infrared microscope. X-ray diffraction (XRD) patterns were obtained using an Empyrean powder diffractometer (Panalytical). 1H and 13C NMR spectra were recorded in DMSO-d6 on 400 MHz NMR spectrometers (Bruker DPX 400). Scanning electron microscopy (SEM) images were taken at 3 kV with a JEOL JSM-7400F system equipped with a Thermo Scientific NORAN System SIX. Ultraviolet-visible spectroscopy (UV-Vis) spectra were recorded using a Cary 100 spectrophotometer. X-ray photoelectron spectroscopy (XPS) was performed on a Thermo Fisher Scientific ESCALAB 250 using monochromated Al Kα X-rays (1486.6 eV). Charging effects were corrected by calibrating the spectra relative to the carbon C1′s peak, positioned at 284.8 eV. Elemental analysis (EA) results were collected using a Thermo Scientific Flash Smart elemental analyzer OEA 2000.
3
FTIR Microspectroscopy of Brain Samples
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The biochemical analysis of brain
samples was performed using FTIR microspectroscopy. The measurements
were performed at the Faculty of Physics and Applied Computer Science
of the AGH University of Science and Technology (Krakow, Poland).
Thermo Scientific Nicolet iN10 MX infrared microscope, equipped with
a ceramic radiation source, was used for the study. For faster scanning
of samples and chemical imaging, the ultrafast mapping system and
a linear array of mercury cadmium telluride (MCT) detectors were used.
In turn, the single spectra from areas of interest were recorded with
the point MCT detector. The samples deposited on CaF2 slides
were analyzed in transmission mode with a spatial resolution of around
25 μm. The spectra were recorded for the wavenumber range 4000–900
cm–1 with spectral resolution set to 8 cm–1. 32 scans were averaged per both sample and background spectrum.
The data acquisition as well as spectral analysis were performed with
OMNIC Picta software (version 8.1).
samples was performed using FTIR microspectroscopy. The measurements
were performed at the Faculty of Physics and Applied Computer Science
of the AGH University of Science and Technology (Krakow, Poland).
Thermo Scientific Nicolet iN10 MX infrared microscope, equipped with
a ceramic radiation source, was used for the study. For faster scanning
of samples and chemical imaging, the ultrafast mapping system and
a linear array of mercury cadmium telluride (MCT) detectors were used.
In turn, the single spectra from areas of interest were recorded with
the point MCT detector. The samples deposited on CaF2 slides
were analyzed in transmission mode with a spatial resolution of around
25 μm. The spectra were recorded for the wavenumber range 4000–900
cm–1 with spectral resolution set to 8 cm–1. 32 scans were averaged per both sample and background spectrum.
The data acquisition as well as spectral analysis were performed with
OMNIC Picta software (version 8.1).
4
Automated Infrared Particle Analysis
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Particle
analyses were conducted on a Nicolet iN10 MX Infrared Microscope (Thermo
Fisher). About 1500 particles were deposited on a metal-coated microscope
slide for particle measurement. Using the self-developed measurement
and analysis software GEPARD,7 (link) an optical
image was acquired using the external side illumination (resembling
dark-field illumination), which was used for automated particle recognition.
For each recognized particle, a rectangular FTIR aperture was calculated
such that the aperture optimally covers the particle without exceeding
its boundary. A maximum aperture size of 150 × 150 μm2 was set to avoid saturated spectra on large particles. For
background spectral acquisition, the needed apertures were grouped
using a 10% area margin, and for each group, a background spectrum
with a square aperture representing the group aperture area was acquired
at an empty spot on the microscopy slide. After acquisition of all
background apertures, the stage was driven to each particle location
and a spectrum was acquired. Background and sample measurements were
conducted in reflection mode at a resolution of 4 cm–1 and 32 scans per acquisition. The sample spectra were background
corrected by calculating −log 10(sample spectrum/background
spectrum).
analyses were conducted on a Nicolet iN10 MX Infrared Microscope (Thermo
Fisher). About 1500 particles were deposited on a metal-coated microscope
slide for particle measurement. Using the self-developed measurement
and analysis software GEPARD,7 (link) an optical
image was acquired using the external side illumination (resembling
dark-field illumination), which was used for automated particle recognition.
For each recognized particle, a rectangular FTIR aperture was calculated
such that the aperture optimally covers the particle without exceeding
its boundary. A maximum aperture size of 150 × 150 μm2 was set to avoid saturated spectra on large particles. For
background spectral acquisition, the needed apertures were grouped
using a 10% area margin, and for each group, a background spectrum
with a square aperture representing the group aperture area was acquired
at an empty spot on the microscopy slide. After acquisition of all
background apertures, the stage was driven to each particle location
and a spectrum was acquired. Background and sample measurements were
conducted in reflection mode at a resolution of 4 cm–1 and 32 scans per acquisition. The sample spectra were background
corrected by calculating −log 10(sample spectrum/background
spectrum).
5
Comprehensive Characterization of Materials
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A PANalytical’s Empyrean diffractometer was employed to conduct X-ray diffraction (XRD) measurements. Fourier transformed infrared (FTIR) spectra were collected on a Thermo Scientific Nicolet iN 10Mx infrared microscope. UV–Vis spectra were recorded on a Cary 100 spectrophotometer equipped with a diffuse reflectance accessory (DRA). An Edinburgh FI/FSTCSPC 920 fluorimeter was used to collect photoluminescence (PL) spectra. The morphologies of the samples were characterized using a scanning electron microscope (SEM, JEOL JSM-7400F equipped with a FEG source) operated at 3.5 kV using a secondary electrons detector. X-ray photoelectron spectroscopy (XPS) measurements were conducted on a Thermo Fisher Scientific ESCALAB 250 using monochromated Kα X-rays (1486.6 eV), and all the binding energies obtained in XPS spectra were calibrated using the C 1s peak at 284.6 eV. N2 (99.999%) adsorption-desorption measurements were performed using a Quantachrome autosorb IQ2 at 77 K. The specific surface area was calculated using the Brunauer–Emmet–Teller (BET) model. Pore‐size distribution was calculated using non‐localized density functional theory (NLDFT) from the nitrogen sorption measurements. The amount of generated gases in the cell of the PEC set up were analyzed using a gas chromatograph (Agilent 7820 GC system) equipped with a thermal conductivity detector (TCD).
6
Felodipine Miscibility in Polymeric Films
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In order to understand the miscibility and diffusivity of felodipine in the polymeric spin coated films and the possibility of drug-polymer interaction, drug particles on uncoated glass coverslips as a control, or spin coated polymer films (Eudragit E PO, PVPVA, Soluplus or HEC) representing miscible, partially miscible or immiscible binary systems respectively were heated at 20 ºC/min from 30-140 ºC and then kept isothermal slightly above the melting point of the drug for 15 minutes to allow for the maximum possible interaction between felodipine and the polymeric films. The samples were then analysed using a Nicolet iN10MX infrared microscope (Thermo Fisher Scientific, Madison, WI, US) with 25 μm spatial resolution in transmission mode using the coverslip glass as a reference. With standard 0.17mm glass coverslips the useable spectral range was from 4000 to 1200 cm -1 . IR maps were acquired with 64 scans at 8 cm -1 spectral resolution, and analysed using the Omnic Picta software (Thermo Fisher)
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