Eagle s mem

Manufactured by Corning

Sourced in United States

Eagle's MEM is a cell culture medium designed for the growth and maintenance of various mammalian cell lines. It provides a balanced salt solution, amino acids, vitamins, and other essential nutrients required for cell proliferation and survival. The medium is commonly used in research and laboratory settings to support the in vitro cultivation of cells.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Bovine insulin, by Merck Group (1 mentions) Mda mb 231 cell, by American Type Culture Collection (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Hbe cells, by American Type Culture Collection (1 mentions) Crystal violet, by Merck Group (1 mentions) Glutaraldehyde, by Merck Group (1 mentions) Glutamine, by Merck Group (1 mentions) Capsaicin, by Merck Group (1 mentions) C fos, by Cell Signaling Technology (1 mentions) Gentamicin, by Merck Group (1 mentions) Phospho jnk, by Cell Signaling Technology (1 mentions) P70s6k, by Cell Signaling Technology (1 mentions) Capsanthin, by Merck Group (1 mentions) β actin antibody, by Merck Group (1 mentions) Phospho p90rsk, by Cell Signaling Technology (1 mentions) Phospho s6, by Cell Signaling Technology (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Modified Eagle’s Medium (MEM; (2 citations)

4 protocols using eagle s mem

Growth and Infectivity of Recombinant VSV

Check if the same lab product or an alternative is used in the 5 most similar protocols

Syrian golden hamster kidney fibroblast cells (BHK-21; ATCC# CCL-10) were grown in monolayer cultures maintained in Minimum Essential Medium (Eagle's MEM, Cellgro) supplemented with 9% fetal bovine serum (FBS, Gibco), 0.3% glucose (w/v), 3.4 mM L-glutamine, 90 U/ml penicillin and 90 µg/ml streptomycin, and kept in a 5% CO₂ atmosphere at 37°C. Infectivity [plaque forming units (PFU) per ml] of virus stocks was determined by standard plaque assay on BHK-21 cells. Recombinant wild-type (wt) VSV (Indiana serotype) was generated previously [46] (link) using pBS-L, pBS-P, pBS-N, and pVSVFL(+) plasmids, and was kindly provided by John K. Rose (Yale University) [47] (link).

Moerdyk-Schauwecker M., Hwang S.I, & Grdzelishvili V.Z. (2014). Cellular Proteins Associated with the Interior and Exterior of Vesicular Stomatitis Virus Virions. PLoS ONE, 9(8), e104688.

+ Open protocol

+ Expand

Culturing Human Breast Cancer and Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human breast carcinoma MDA-MB-231 cells (ATCC) were maintained in Eagle’s MEM (Cellgro) supplemented with 10% FBS (Atlanta Biologicals), 2 mM HEPES (Gibco), and 6 ng/mL bovine insulin (Sigma). HBE cells (ATCC) were cultured in MEM supplemented with 10% FBS. All mammalian cells were incubated at 37°C in a humidified incubator containing 5% CO₂.

Shen T., Chen X.M., Harder B., Long M., Wang X.N., Lou H.X., Wondrak G.T., Ren D.M, & Zhang D.D. (2014). Plant Extracts of the Family Lauraceae: A Potential Resource for Chemopreventive Agents that Activate the Nuclear Factor-Erythroid 2-Related Factor 2/Antioxidant Response Element Pathway. Planta medica, 80(5), 426-434.

+ Open protocol

+ Expand

Activation of Cellular Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dihydrocapsaicin was obtained from Cayman Chemical (Ann Arbor, MI, USA). Capsiate, capsanthin, capsaicin, 12-O-tetradecanoylphorbol 13-acetate (TPA), glutaraldehyde, crystal violet, glutamine, gentamicin, and β-actin antibody were purchased from Sigma-Aldrich (St. Louis, MO, USA). Eagle’s MEM was purchased from Corning (New York, NY, USA). Antibodies for phospho-Akt, phospho-p38, phospho-JNK, phospho-p90RSK, phospho- phospho-p70S6K, phospho-S6, Akt, p70S6K, c-Fos, and p38 were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies to detect phosphorylated ERK1/2, ERK1/2, and RSK2 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Lee J.S., Kim Y.A., Jang Y.J., Oh Y, & Byun S. (2019). Dihydrocapsaicin Inhibits Epithelial Cell Transformation through Targeting Amino Acid Signaling and c-Fos Expression. Nutrients, 11(6), 1269.

+ Open protocol

+ Expand

Cell Culture and Animal Study Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

MDA-MB-468 cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and were maintained in Leibovitz’s L-15 medium (Corning, Manassas, VA, USA), and supplemented with 10% fetal bovine serum and antibiotics penicillin (100 µg/ml) and streptomycin (100 µg/ml) without CO₂. HT1080-Luc2 cells obtained from Caliper (Hopkinton, MA, USA) were grown at 37 °C with 5% CO₂ in Eagle's MEM from Corning (Manassas, VA, USA), and supplemented with 10% fetal bovine serum (Seradigm, Radnor, PA, USA) and antibiotics penicillin (100 µg/ml) and streptomycin (100 µg/ml) (Invitrogen, Carlsbad, CA, USA).
All animal studies were performed in compliance with the approved animal protocols and guidelines of the Institutional Animal Care and Use Committee of Weill Cornell Medicine. BALB/c Nu/Nu female nude mice (5–6 weeks) were purchased from Charles River (Wilmington, MA).

Tung C.H., Han M.S., Kim Y., Qi J, & O’Neill B.E. (2017). Tumor ablation using low-intensity ultrasound and sound excitable drug. Journal of controlled release : official journal of the Controlled Release Society, 258, 67-72.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!