hiPSCs were induced to differentiate into endoderm (phase II) using 1% Penicillin-Streptomycin Solution (×100) (16823191: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 100 ng/mL Recombinant Human/Mouse/Rat Activin A Protein (338-AC-010: R&D Systems, Minneapolis, MN) in RPMI 1640 with L-Gln, liquid medium (30264–85: Nacalai Tesque, Kyoto, Japan), supplemented with 0.2% KSR (10828028: ThermoFisher Scientific, Tokyo, Japan) for 24 h.
Recombinant human mouse rat activin a protein
Manufactured by R&D Systems
Sourced in Japan
Recombinant Human/Mouse/Rat Activin A Protein is a recombinant protein that corresponds to the mature form of human, mouse, or rat Activin A. Activin A is a member of the transforming growth factor-beta (TGF-β) superfamily and plays a role in a variety of cellular processes, including cell growth, differentiation, and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin solution 100, by Fujifilm (1 mentions)
Ab281583, by Abcam (1 mentions)
Anti rabbit igg, by Cell Signaling Technology (1 mentions)
Smad3 c67h9 rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase hrp linked anti mouse igg, by Cell Signaling Technology (1 mentions)
Sb431542, by Merck Group (1 mentions)
Forskolin, by Fujifilm (1 mentions)
3 protocols using recombinant human mouse rat activin a protein
1
Directed Differentiation of hiPSCs into Endoderm
2
Antibody Characterization for TGF-β Pathway
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Anti-fibronectin rabbit polyclonal antibody (ab2413) and anti-CD31 rabbit recombinant multiclonal antibody (ab281583) were purchased from Abcam (Cambridge, MA); SMAD2 (L16D3) mouse monoclonal antibody (# 3103), phospho-SMAD2 (138D4) rabbit monoclonal antibody (# 3108), SMAD3 (C67H9) rabbit monoclonal antibody (# 9523), phospho-SMAD3 (C25A9) rabbit monoclonal antibody (# 9520), SMAD4 (D3M6U) rabbit monoclonal antibody (# 38454), horseradish peroxidase (HRP)-linked anti-mouse IgG (# 7076) and anti-rabbit IgG (# 7074) were purchased from Cell Signaling (Beverly, MA); mouse monoclonal antibody HLA-G was purchased from EXBIO (#11-499-C100) ; cytokeratin 7-specific rabbit polyclonal antibody (# 17513-1-AP) was purchased from Proteintech (Wuhan, China); mouse monoclonal anti-α-Tubulin antibody (sc-23948) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA); mouse monoclonal anti-cytokeratin 8 antibody (GB12233-100) was obtained from Servicebio (Wuhan, China); anti-Mouse Secondary Antibody, Alexa Fluor 488 (#A11001), anti-Rabbit Secondary Antibody, Alexa Fluor 594 (# A11012) and ACVR1B (ALK4) TaqMan primers (# 433182) were obtained from Thermo Fisher (Waltham, MA); recombinant human/mouse/rat activin A protein was provided by R&D (Minneapolis, MN); SB431542 (TGF-beta type I receptor inhibitor) (# S4317) was purchased from Sigma Aldrich (St. Louis, MO).
3
Differentiating PFG Cells to Liver Buds
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To further differentiate PFG to liver bud progenitors, one of two related types of differentiation conditions were used on days 4–6 of differentiation using 10 ng/mL Recombinant Human/Mouse/Rat Activin A Protein (338-AC-010: R&D Systems, Minneapolis, MN), 30 ng/mL Bone Morphogenetic Protein 4 (truncated) (BMP-4), Human, recombinant, Animal-derived-free (020–18851: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan), 1 mM 8-Bromo-cAMP, sodium salt (76939-46-3: R&D Systems, Minneapolis, MN) or 1 μM Forskolin (067–02191: FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) in CDM3 medium for 3 days (day 4 to 6).
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