GSK’963 (Aobious), GSK’2982772, Necrostatin-1 and Necrostatin-1s (Nec-1 and Nec-1s; Selleck Chemicals) were dissolve in 100% DMSO at 10 mg/ml and kept at -20°C for long-term storage. Ibuprofen sodium (Sigma-Aldrich) was prepared at 5 mg/ml in deionized water and stored at 4°C for no longer than 1 week.
Gsk2982772
Manufactured by Selleck Chemicals
Sourced in United States
GSK2982772 is a chemical compound that can be used as a laboratory reagent. It functions as a selective inhibitor of a specific enzymatic target. Further details about its core function or intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
Necrostatin 1, by Selleck Chemicals (2 mentions)
Tnf α, by Thermo Fisher Scientific (2 mentions)
Nec 1, by Selleck Chemicals (1 mentions)
Ibuprofen sodium, by Merck Group (1 mentions)
Gsk 963, by Selleck Chemicals (1 mentions)
Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Sb225002, by Selleck Chemicals (1 mentions)
Viper, by Novus Biologicals (1 mentions)
Lipopolysaccharides (lps), by Novus Biologicals (1 mentions)
Tnf α, by Selleck Chemicals (1 mentions)
Sc144, by Selleck Chemicals (1 mentions)
Interleukin 6 (il 6), by Selleck Chemicals (1 mentions)
Bx795, by MedChemExpress (1 mentions)
Mrt67307, by Selleck Chemicals (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
4 protocols using gsk2982772
1
Preparation of Pharmacological Inhibitors
2
Inflammatory Factors Modulate MUC16 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 1x106 HEY cells were first treated with 10, 50 and 100 ng/ml LPS (Sigma-Aldrich; Merk KGaA), IL-6 (PeproTech, Inc.) or IL-8 (PeproTech, Inc.), or 2.5, 10 and 25 ng/ml of TNF-α (PeproTech, Inc.) for 24, 48 and 72 h at 37˚C, with 1X PBS used as a control. The lowest concentration and shortest stimulation time of inflammation-associated factors resulting in a statistically significant change in MUC16 mRNA expression levels were selected as the optimal concentration and duration, respectively. HEY cells were treated with LPS, IL-6, IL-8 or TNF-α at the optimal concentration and for the optimal duration. For co-treatment,HEY cells were treated with 10 ng/ml LPS combined with 500 nM Toll-like receptor 4 (TLR4) antagonist VIPER (Novus Biologicals, LLC) for 48 h, 50 ng/ml IL-6 was combined with 10 µM membrane glycoprotein 130 (gp130) inhibitor SC144 (Selleck Chemicals) for 24 h, 50 ng/ml IL-8 was combined with 10 µM CXCR2 antagonist SB225002 (Selleck Chemicals) for 48 h or 2.5 ng/ml TNF-α was combined with 100 nM of its inhibitor GSK2982772 (Selleck Chemicals) for 24 h.
3
Modulation of BMDM Activation Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BMDMs were stimulated with MRT67307 (Selleckchem, cat no. S7948), BX795 (Medchemexpress, cat no. HY-10514), 10 μM GSK2982772 (Selleckchem, cat no. S8484), or 20μM Necrostatin-s1 (Selleckchem, cat no. S8641) for the indicated times in the presence of 20 ng/ml mM-CSF.
4
Investigating Cell Line Responses to Small Molecules
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Huh7 and SK-HEP-1 cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea). Dulbecco’s modified Eagle medium, RPMI-1640 medium, fetal bovine serum, L-glutamine, penicillin, and streptomycin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Sulfasalazine, ferrostatin-1, necrostatin-1, deferoxamine, and 1-methyl-D-tryptophan were purchased from Merck KGaA (Darmstadt, Germany). Erastin, RSL3, necrostatin-1s, and GSK2982772 and Z-VAD-FMK were obtained from Selleckchem (Houston, TX, USA). TNFα was supplied from PeproTech (Cranbury, NJ, USA) and SM164 was obtained from AdooQ Bioscience (Irvine, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!