The following antibodies were used for cell surface staining: anti-mouse CD4-Alexa700 (clone GK1.5), CD8-APC-eFluor780 (clone 53–6.7), CD25-eFluor450 or PerCPCy5.5 (clone PC61.5), Vα2TCR-PerCP eFluor710 or APC (clone B20.1), Vα3.2TCR-FITC (clone RR3-16), CD45.1-PE-Cy7 (clone A20), CD45.2-FITC (clone 104) (eBioscience), or CD4-redfluor710, CD8-APC-Cy7 (Tonbo Bioscience), and Neuropilin-1-PE (clone 3E12) (Biolegend). Cell proliferation dye eFluor450 staining was performed at 2 μM for 10 min at 37 °C (eBioscience). For intracellular staining: Nur77-PE (clone 12.14), and Foxp3-PE (clone FJK-16s) or -APC were used (eBioscience). Cells were first surface stained for 15 min on ice, followed by fixation and permeabilization according to manufacturer’s instructions using Foxp3 Transcription Factor staining buffer set (eBioscience). Flow cytometry was performed using a BD Fortessa or Fortessa X20 analyzer (BD Bioscience), and data were analyzed using FlowJo software (Treestar).
Cd45.2 fitc clone 104
Manufactured by Thermo Fisher Scientific
CD45.2-FITC (clone 104) is a fluorochrome-conjugated monoclonal antibody that specifically binds to the CD45.2 isoform of the mouse CD45 antigen. CD45 is a receptor-linked protein tyrosine phosphatase that is expressed on the surface of most hematopoietic cells. The CD45.2 isoform is expressed on a subset of mouse lymphocytes.
Automatically generated - may contain errors
Lab products found in correlation
Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions)
Fortessa, by BD (2 mentions)
Anti mouse cd4 alexa700, by Thermo Fisher Scientific (2 mentions)
Cd8 apc efluor780 clone 53, by Thermo Fisher Scientific (2 mentions)
Cd25 efluor450 or percpcy5.5 clone, by Thermo Fisher Scientific (2 mentions)
Vα2tcr percp efluor710 or apc, by Thermo Fisher Scientific (2 mentions)
Vα3.2tcr fitc clone rr3 16, by Thermo Fisher Scientific (2 mentions)
Cd45.1 pe cy7 clone a20, by Thermo Fisher Scientific (2 mentions)
Neuropilin 1 pe clone 3e12, by BioLegend (2 mentions)
Nur77 pe clone 12.14, by Thermo Fisher Scientific (2 mentions)
2 protocols using cd45.2 fitc clone 104
1
Multiparameter Flow Cytometry Immunophenotyping
2
Multiparameter Flow Cytometry Immunophenotyping
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The following antibodies were used for cell surface staining: anti-mouse CD4-Alexa700 (clone GK1.5), CD8-APC-eFluor780 (clone 53–6.7), CD25-eFluor450 or PerCPCy5.5 (clone PC61.5), Vα2TCR-PerCP eFluor710 or APC (clone B20.1), Vα3.2TCR-FITC (clone RR3-16), CD45.1-PE-Cy7 (clone A20), CD45.2-FITC (clone 104) (eBioscience), or CD4-redfluor710, CD8-APC-Cy7 (Tonbo Bioscience), and Neuropilin-1-PE (clone 3E12) (Biolegend). Cell proliferation dye eFluor450 staining was performed at 2 μM for 10 min at 37 °C (eBioscience). For intracellular staining: Nur77-PE (clone 12.14), and Foxp3-PE (clone FJK-16s) or -APC were used (eBioscience). Cells were first surface stained for 15 min on ice, followed by fixation and permeabilization according to manufacturer’s instructions using Foxp3 Transcription Factor staining buffer set (eBioscience). Flow cytometry was performed using a BD Fortessa or Fortessa X20 analyzer (BD Bioscience), and data were analyzed using FlowJo software (Treestar).
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