Minispec bca analyzer lf110

All experiments were performed in accordance with the animal ethics laws of Saxony, Germany, and were approved by the state animal ethics committee (Landesdirektion Sachsen, Leipzig, Germany). Male C57BL/6N mice were put on a WD (Ssniff Spezialdiäten, D12331) or control chow diet (Ssniff Spezialdiäten, V1534) starting at ~8 weeks of age. Animals were maintained on the respective diets for 3 months and weighed weekly. For the ‘reversion’ experiments, animals maintained on a WD for 3 months were changed to a chow diet and analyzed 1 or 3 months later. Body composition was measured using the Minispec BCA Analyzer LF110 (Bruker Biospin). Mice were maintained on a 12-h day/12-h night cycle. Water and food were provided ad libitum. The temperature was maintained at 22 ± 2 °C, with humidity maintained at 55 ± 10%.

All experiments were performed in accordance with the animal ethics laws of Saxony, Germany, and were approved by the state animal ethics committee (Landesdirektion Sachsen, Leipzig, Germany). Male C57BL/6N mice were put on a WD (Ssniff Spezialdiäten, D12331) or control chow diet (Ssniff Spezialdiäten, V1534) starting at ~8 weeks of age. Animals were maintained on the respective diets for 3 months and weighed weekly. For the ‘reversion’ experiments, animals maintained on a WD for 3 months were changed to a chow diet and analyzed 1 or 3 months later. Body composition was measured using the Minispec BCA Analyzer LF110 (Bruker Biospin). Mice were maintained on a 12-h day/12-h night cycle. Water and food were provided ad libitum. The temperature was maintained at 22 ± 2 °C, with humidity maintained at 55 ± 10%.