Masson trichrome staining kit

Lung tissue was fixed with 4% polyformaldehyde solution for 24 h, dehydrated and paraffin embedded and cut into 4-μm slices. Then, the slices (3 slices per mouse) were stained with hematoxylin and eosin (H&E) to observe histopathological changes in the colon under a microscope. Masson trichrome staining was performed using Masson trichrome staining kit (Beyotime, China). Histological sections were stained with hematoxylin and eosin (H&E) to assess histopathological changes, and Masson trichrome staining was performed following the manufacturer's instructions.

Rat hearts were collected, and myocardial tissue was washed with normal saline. Then, we used 4% paraformaldehyde to fix myocardial tissue. We used gradient alcohol for dehydration and embed myocardial tissue in paraffin. Then, we used a microtome to make paraffin blocks into paraffin sections. Before hematoxylin-eosin (HE) staining, we put paraffin slices in xylene and gradient alcohol for dewaxing and hydration. Then, we stained the cell nucleus with hematoxylin solution (Beyotime, Shanghai, China) for 1 minute. After rinsing slices with running water, we used 1% hydrochloric acid alcohol for differentiation. Then, we used eosin solution (Beyotime, Shanghai, China) to stain the cytoplasm. Finally, we used neutral gum for sealing. In addition, we used Masson trichrome staining kit (Beyotime, Shanghai, China) for staining according to the manufacturer's instructions. Collagen fibers appear blue and muscles appear red.