Human lung microvascular endothelial cells (HMVEC) were serum starved overnight in EBM-2 basal medium (Lonza, Basel, Switzerland) at 37 °C prior to seeding onto Geltrex at a concentration of 2 × 104 cells/well within a 15 well μ-Angiogenesis slide with or without the addition of TSP-1 (1500 ng/ml) in EBM-2 basal medium containing EGM-2 MV SingleQuot Kit supplements and growth factors (Lonza, Basel, Switzerland). After 6 h, images of the angiogenesis formation were obtained using the EVOS XL Cell Imaging System. Angiogenesis formation was determined by blindly counting the vessel-like structures formed within each well with the use of ImageJ software.
Tsp 1
Manufactured by Lonza
The TSP-1 is a laboratory equipment designed for the separation and purification of biological samples. It utilizes a unique separation principle to effectively isolate target molecules or cells from complex mixtures. The core function of the TSP-1 is to enable efficient and reliable sample preparation for downstream analytical or research applications.
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2 protocols using tsp 1
1
Angiogenesis Assay in HMVEC
2
Angiogenesis Assay of Human Lung Microvascular Endothelial Cells
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Human lung microvascular endothelial cells (HMVEC) were serum starved overnight in EBM-2 basal medium (Lonza, Basel, Switzerland) at 37˚C prior to seeding onto Geltrex at a concentration of 2 x 10 4 cells/well within a 15 well µ-Angiogenesis slide with or without the addition of TSP-1 (1500 ng/ml) in EBM-2 basal medium containing EGM-2 MV SingleQuot Kit supplements and growth factors (Lonza, Basel, Switzerland). After 6 hours, images of the angiogenesis formation were obtained using the EVOS XL Cell Imaging System. Angiogenesis formation was determined by blindly counting the vessel-like structures formed within each well with the use of ImageJ software.
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