MDA-MB-231 and MDA-MB-468 cells were plated at 250 cells per well in 96-well ultralow attachment plates (Corning, Corning, NY) in 100 μL of MammoCult™ human media (STEMCELL Technologies, Vancouver, Canada). Twenty-four hours after being plated, cells received radiation and/or deferoxamine depending on the experimental group. Tumorsphere formation was evaluated using an EVOS FLc microscope (Thermo Fisher Scientific, Carlsbad, CA) 7 days after therapeutic dosing and reported as sphere number.
Mammocult human media
Manufactured by STEMCELL
Sourced in Canada
MammoCult™ is a serum-free and animal component-free medium designed for the culture of human mammary epithelial cells, including primary breast cancer cells and human breast cell lines. It supports the growth and differentiation of these cells in vitro.
Automatically generated - may contain errors
2 protocols using mammocult human media
1
Tumorsphere Formation Assay in Breast Cancer
2
Vitamin K Modulates Stem Cell Phenotype
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The impact of vitamin K compounds on the stem cell phenotype was assessed with mammosphere assays. Hs578T (6,000 cells/well) and SUM159PT (4,000 cells/well) cells were plated in MammoCult Human Media (STEMCELL Technologies, Vancouver, CA) with added heparin and hydrocortisone in 6-well low attachment plates. Cells were treated with K1, K2, K1-EPO, or vehicle in the presence or absence of 2 μM warfarin. Mammospheres were imaged after 8 days on an IN Cell Analyzer 2200 (GE Healthcare) and counted manually or with NIST’s Integrated Colony Enumerator software (Gaithersburg, MD).
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