Prlb00

Commercially available ELISA kits for measuring serum HMGB1 (#SEKR-0074, Solarbio Science & Technology Co., Ltd., Beijing, China), interleukin-1β (IL-1β, #PRTA00, R&D System, Minneapolis, MN, USA), IL-6 (#PR6000B, R&D System), and tumor necrosis factor-α (TNF-α, #PRLB00, R&D System) levels and dorsal hippocampal IL-1β, IL-6, and TNF-α levels were used according to the manufacturers’ instructions. Briefly, blood samples were collected transcardially under anesthesia with an overdose of sevoflurane (5% for 5 min) and centrifuged at 3,000 g for 10 min at 4 °C; then, the serum was collected. Concurrently, the dorsal hippocampus (dHPC) was dissected based on the atlas of Paxinos and Watson [38 ] and homogenized in radioimmunoprecipitation (RIPA) lysis buffer followed by centrifugation at 12,000g for 10 min at 4 °C. The supernatant was collected, and the protein concentration was determined using a bicinchoninic acid (BCA) assay kit (Beyotime Biotechnology, Shanghai, China). All serum and tissue samples were used to detect the concentrations of HMGB1 and proinflammatory cytokines, and the absorbance was quickly read using an ELISA microplate reader as described in our previous study [39 (link)].

Quantikine® ELISA kits for IL-1β (RLB00, SRLB00, PRLB00), IL-6 (R6000B, SR6000B, PR6000B), and TNF-α (RTA00, SRTA00, PRTA00) were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). RvD₁ (Cat# 500,380) was purchased from Cayman Chemical. (Ann Arbor, Michigan, USA). RvE₁ (Cat# MBS2604861) and soluble receptor of AGE (sRAGE) (Cat# MBS722302) were purchased from MyBioSource, Inc. (San Diego, CA, USA), and LxA₄ (Cat# 407,010) was purchased from Neogen Corporation (Lexington, Kentucky, USA). All measurements were performed according to the kit manufacturer’s instructions.