Antibodies against gapdh mb001

SPARC antibody (#5420) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Goat anti-rabbit immunoglobulin G (IgG)-horseradish peroxidase secondary antibodies (BS10003) and antibodies against GAPDH (MB001) were purchased from Bioworld Technology, Inc. (St. Louis Park, MN, USA).

Antibodies against CCR9 (ab38567, 1:500 dilution) were obtained from Abcam. Antibodies against the following proteins were purchased from Cell Signaling Technology: MEK1/2 (9122, 1:1000 dilution), phosphorylated (p) MEK1/2Ser32/36 (9154, 1:1000 dilution), ERK1/2 (4695, 1:1000 dilution), p‐ERK1/2Thr202/Tyr204 (4370, 1:1000 dilution), JNK (9252, 1:1000 dilution), p‐JNKThr183/Tyr185 (4668, 1:1000 dilution), P38 (9212, 1:1000 dilution), p‐P38Thr180/Tyr182 (4511, 1:1000 dilution), AKT (4691, 1:1000 dilution), p‐AKTSer473 (4060, 1:1000 dilution), glycogen synthase kinase 3β (GSK3β; 9315, 1:1000 dilution), p‐GSK3βSer9 (9322, 1:1000 dilution), p70S6 kinase (2708, 1:1000 dilution), p‐p70S6 kinaseSer371 (9208, 1:1000 dilution), mTOR (2983, 1:1000 dilution), p‐mTORSer2448 (2971, 1:1000 dilution), PI3 kinase p85 (4257, 1:1000 dilution), and p‐PI3 kinase p85Tyr458 (4228, 1:1000 dilution). Antibodies against β‐myosin heavy chain (sc53090, 1:200 dilution) and atrial natriuretic peptide (sc20158, 1:200 dilution) were purchased from Santa Cruz Biotechnology. Antibodies against GAPDH (MB001, 1:10 000 dilution) were obtained from Bioworld Technology. The BCA protein assay kit was purchased from Pierce (Thermo Fisher Scientific). We used peroxidase‐conjugated secondary antibodies (1:10 000 dilution; Jackson ImmunoResearch Laboratories) for visualization.