DMG-PEG2000 (SUNBRIGHT® GM-020), DOPC (COATSOME® MC-8181), and SS-OP (COATSOME® SS-OP) were obtained from NOF CORPORATION (Tokyo, Japan). Cholesterol was purchased from Nacalai Tesque, Inc. (Kyoto, Japan); 1,2-Distearoyl-sn-glycerol-3- phosphocholine (DSPC) from Avanti Polar Lipids, Inc. (Alabaster, AL); DLin-MC3-DMA (MC3) from MedChemExpress (Monmouth Junction, NJ); DL-malic acid from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan); NucleoSpin Plasmid Transfection Grade from MACHEREY-NAGEL GmbH & Co. (KG, Germany); Sap I restriction enzyme and HiScribe T7 High Yield RNA Synthesis Kit from New England BioLabs, Inc.(Beverly, MA, USA); DNase TURBO from Life Technologies (Carlsbad, CA); CleanCap® Reagent AG from TriLink BioTechnologies (San Diego, CA); 2-(N-morpholino)ethanesulfonic acid (MES) from DOJINDO LABORATORIES (Kumamoto, Japan); and Quanti-iTTM RiboGreen RNA reagent from Molecular Probes (Eugene, OR).
2 n morpholino ethanesulfonic acid mes
Manufactured by Dojindo Laboratories
Sourced in Japan
2-(N-morpholino)ethanesulfonic acid (MES) is a zwitterionic biological buffer compound. It is commonly used to maintain a stable pH in various biological and biochemical applications.
Automatically generated - may contain errors
Lab products found in correlation
Cleancap reagent ag, by TriLink (1 mentions)
Dlin mc3 dma mc3, by MedChemExpress (1 mentions)
Cholesterol, by Nacalai Tesque (1 mentions)
Turbo dnase, by Thermo Fisher Scientific (1 mentions)
Nucleospin plasmid transfection grade, by Macherey-Nagel (1 mentions)
Gelatin, by Fujifilm (1 mentions)
Collagenase, by Fujifilm (1 mentions)
Penicillin streptomycin 100, by Thermo Fisher Scientific (1 mentions)
D camphor 10 sulfonic acid, by Fujifilm (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Mannitol, by Fujifilm (1 mentions)
Trypsin inhibitor, by Fujifilm (1 mentions)
1 3 5 benzenetricarboxylic acid, by Fujifilm (1 mentions)
2 protocols using 2 n morpholino ethanesulfonic acid mes
1
Lipid-based mRNA Delivery Formulation
2
Endothelial Cell Culture and Characterization
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The following reagents were used: endothelial cell basal medium (EBM-2) and EGM-2 Single Quots (supplemental factors) (Lonza Japan Ltd., Tokyo, Japan); Dulbecco's modified Eagle's medium (DMEM), penicillin/streptomycin (100 ×), and trypsin-EDTA (Invitrogen/GIBCO, Grand Island, NY, USA); tissue-culture treated cell culture dishes/plates (Corning Inc., Tokyo, Japan); collagenase, trypsin inhibitor, mannitol, D-camphor-10-sulfonic acid (CSA), 1,3,5-benzenetricarboxylic acid (Trimesate), and gelatin (Wako Pure Chemical Industries Ltd., Osaka, Japan); 2-(N-morpholino) ethanesulfonic acid (MES) (Dojindo, Kumamoto, Japan); growth factor reduced (GFR) Engelbreth-Holm-Swarm (EHS) gel (BD Biosciences, Bedford, MA, USA); and acetaminophen (p-Acetamidophenol) (Nacalai Tesque Inc., Kyoto, Japan). All reagents used in the present study were of analytical grade.
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