Phosphate dibasic trihydrate, sodium hydroxide, sodium chloride, glacial acetic acid (Mosreaktive, Russia); 2,6-dichlorophenolindophenol sodium salt, Nafion 117 (5% in a mixture of lower aliphatic alcohols and water), graphene/PEDOT:PSS hybrid ink, PEDOT:PSS, low-molecular-weight chitosan, potassium hexacyanoferrate(III) (Sigma, Burlington, MA, USA); sorbitol, glucose, yeast extract, bacteriological agar-agar, potassium chloride (Dia-M, Russia) were used. Three-contact screen-printed electrodes (SPE) were purchased from Color Electronics (Moscow, Russia).
Gluconobacter oxydans sbsp. industrius VKM B-1280 (All-Russian Collection of Microorganisms) strain was used as bioreceptor. Cells were grown according to the method described in [24 (link)]. After the cultivation, the G. oxydans cell suspension was washed with phosphate buffer and diluted to a concentration of 0.5 mg wet weight per µL. This suspension was further used to form the bioreceptor.
Gluconobacter oxydans sbsp. industrius VKM B-1280 (All-Russian Collection of Microorganisms) strain was used as bioreceptor. Cells were grown according to the method described in [24 (link)]. After the cultivation, the G. oxydans cell suspension was washed with phosphate buffer and diluted to a concentration of 0.5 mg wet weight per µL. This suspension was further used to form the bioreceptor.