Imager m2 compound microscope

Animals were generally immobilized in a drop of M9 solution with 1 mM levamisole on a 4% agar pad or 10% agarose pad. Most fluorescence images were collected with a 63× oil immersion objective using a Zeiss LSM800 confocal microscope. Representative head neurons/nerve ring images are shown in 10-slice z-stack maximum projection at 0.5μm interval centered on middle of pharynx prepared using Fiji (ImageJ). Germ line images are presented as middle 5-slice z-stack maximum projection at 0.5μm interval. All embryo images are shown as single slice where the nucleus shows the biggest area. Soma and membrane regions were quantified by averaging the mean grey value from three regions of interest (ROIs). Images of ventral cord neurons expressing both WDR-37::mNG and PACS-1::mSc were collected with a 63× oil immersion objective using a Zeiss LSM900 airyscan microscope. For pacs-1(ju2014) rescue experiments, images of head neurons expressing WDR-37::mNG were collected with a 63× oil immersion objective on a Zeiss Axio Imager M2 compound microscope under identical settings.