Micro fine syringe

Manufactured by BD

The Micro-Fine syringe is a laboratory equipment designed for precise fluid handling. It features a fine needle tip and a compact size for accurate and controlled delivery of small volumes of liquids or solutions. The core function of the Micro-Fine syringe is to enable precise and controlled fluid transfer in various laboratory applications.

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Lab products found in correlation

Ganetespib, by Adooq (1 mentions) Cremophor rh40, by Merck Group (1 mentions) Matrigel, by Corning (1 mentions) Nmri foxn1 nu nu mice, by Janvier Labs (1 mentions)

4 protocols using micro fine syringe

Evaluating USP22 in Tumor Growth

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Immunodeficient SCID mice were housed in a controlled, germ-free environment. Animals were randomized into treatment groups and anesthetized by isoflurane inhalation (2–3%, Forene) and HCT116 USP22^+/+ and USP22^−/− cells (1.0 × 10⁶ cells in Corning® Matrigel®) were injected subcutaneously into the left and right flank, respectively, using a 0.3 ml Micro-Fine syringe (BD Bioscience). The bodyweight, as well as the size of the growing tumors, were monitored daily. After the tumors reached a volume of approximately 100 mm³, 25 mg/kg Ganetespib (AdooQ) or vehicle solution (10% DMSO (v/v), 20% (v/v) pre-warmed Cremophor RH40 (55 °C; Sigma-Aldrich), 5% (w/v) dextrose) was injected i.v. for three consecutive days and after 4 days break, for 3 further days. Experiments were performed with six replicates per group in order to assure reproducibility. Experiments were performed blinded during tumor size measurement and subsequent immunohistochemical staining.

Kosinsky R.L., Helms M., Zerche M., Wohn L., Dyas A., Prokakis E., Kazerouni Z.B., Bedi U., Wegwitz F, & Johnsen S.A. (2019). USP22-dependent HSP90AB1 expression promotes resistance to HSP90 inhibition in mammary and colorectal cancer. Cell Death & Disease, 10(12), 911.

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Quantifying Allergen-Induced Neutrophil Influx

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Grass pollen extract (ALK-ABELLO) was injected intradermally into the left volar forearm of grass pollen-allergic individuals according to the manufacturer´s instructions. The diluent was administered into the right volar forearm as negative control. All individuals developed immediate reactions within 15 min. Provided the individuals developed visible cutaneous LPR, skin suction blisters were formed on top of the injection sites after 20-24 h by using a low pressure instrument from Electronic Diversities (Ridge Road, Finksburg, MD, USA) by mounting 2 sterile, 5-hole (5 mm diameter per hole) skin suction plates. Low pressure (150–200 mmHg) was applied over a time period of 2 h, and adjusted according to blister size to prevent blood vessel trauma through excessive mechanical stress. Fluids from intact and hemoglobin-free blisters were collected using a Micro-Fine syringe (BD Biosciences), pooled, centrifuged at 1000 x g for 5 min at 4°C, and analyzed by flow cytometry. The total numbers of neutrophils detected in the blister fluids after injection of allergen ranged from 282-2646. No neutrophils were detected in blister fluids after injection of the diluent.

Polak D., Hafner C., Briza P., Kitzmüller C., Elbe-Bürger A., Samadi N., Gschwandtner M., Pfützner W., Zlabinger G.J., Jahn-Schmid B, & Bohle B. (2018). A novel role for neutrophils in IgE-mediated allergy: evidence for antigen-presentation in late-phase reactions. The Journal of allergy and clinical immunology, 143(3), 1143-1152.e4.

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Xenograft Mouse Model for 4SC-202 Efficacy

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For each animal, one million tumor cells were resuspended in 20 μl of a 1:1 mixture of DMEM medium and BD Matrigel Matrix High Concentration (HC), Growth Factor Reduced (GFR) (BD Bioscience) and kept on ice until transplantation. Nine week old virgin NMRI foxn1nu/nu mice (Janvier Labs) were anesthetized by isoflurane inhalation (2–3%, Forene). The cell suspensions were injected under sterile conditions subcutaneously with a 0.3 ml Micro-Fine syringe (BD Bioscience) into the left abdominal flank. After tumors were palpable (size = 100 mm³), mice were randomly divided into two groups (n = 12 per group) as control and treated. Animals were treated with either vehicle (methylcellulose) or 4SC-202 (120 mg/kg in methylcellulose) for 4 days (b.i.d) via oral gavage and then followed for another week until sacrificing. Mouse weight and tumor size were measured daily.

Mishra V.K., Wegwitz F., Kosinsky R.L., Sen M., Baumgartner R., Wulff T., Siveke J.T., Schildhaus H.U., Najafova Z., Kari V., Kohlhof H., Hessmann E, & Johnsen S.A. (2017). Histone deacetylase class-I inhibition promotes epithelial gene expression in pancreatic cancer cells in a BRD4- and MYC-dependent manner. Nucleic Acids Research, 45(11), 6334-6349.

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Suction Blister Fluid Collection Protocol

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SBF was collected by the method developed by Kiistala (15 ). Suction blisters were created on the thigh of each participant as a site easily accessible when wearing shorts and one that can be discretely covered for cosmetic reasons until suction blisters have fully healed. The skin site was first thoroughly disinfected with an alcohol swab. Suction was then applied using a Negative Pressure Cutaneous Suction System (NPV-2, Electronic Diversities). Sterile orifice plates with 3 holes (8 mm diameter each) along with the suction cup were firmly attached to the skin using straps. Suction at −50 to −70 kPa (gauge) was applied at 40°C for ~45 min until blister formation was complete. Fluid from intact and hemoglobin-free blisters (~ 50 μL per blister) was collected using a Micro-Fine syringe (BD Biosciences) and stored in Protein lo-bind Eppendorf tubes at −80°C.

Samant P.P., Niedzwiecki M.M., Raviele N., Tran V., Mena-Lapaix J., Walker D.I., Felner E.I., Jones D.P., Miller G.W, & Prausnitz M.R. (2020). Sampling interstitial fluid from human skin using a microneedle patch. Science translational medicine, 12(571), eaaw0285.

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