Anti cd68 sc 20060

Histological reagents, and secondary antibodies were from Sigma (St. Louis, MO, USA). DAB substrate was from Dako (Carpinteria, CA, USA). ECL was from GE Life Sciences (Barcelona, Spain). Anti-MMP-13 (sc-101564), anti-MMP-9 (sc-13520), anti-EMMPRIN (sc-71038), and anti-CD68 (sc-20060) primary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Nanoparticle components were form Avanti Polar Lipids (LabClinics, Barcelona, Spain).

In this study, we used the following antibodies: anti-MCT4 (Cat No. 22787-1-AP, Proteintech, USA; abs124388, absin, China), anti-MCT1 (abs 120479, absin, China), anti-α-actinin (sc-17829, Santa Cruz, USA), anti-BNP (13299-1-AP, Proteintech, USA), anti-NPPA (27426-1-AP, Proteintech, USA), anti-Pan Kla (PTM-1401RM, PTM, China), anti-H3K18La (PTM-1406RM, PTM, China), anti-H4K12La (PTM-1411RM, PTM, China), anti-CD68 (sc-20060, Santa Cruz, USA), anti-iNOS (#13120, CST, USA), anti-Arg-1 (#93668, CST, USA), anti-HIF-1α (#14179, CST, USA), anti-IL-1β (#12242, CST, USA), anti-Mitofilin (ab110329, Abcam, USA), and anti-dsDNA (HYB331-01, Santa Cruz, USA). For immunoblotting experiments, horseradish peroxidase (HRP)-labeled Goat anti-mouse IgG (A0216, Beyotime, China) and goat anti-rabbit IgG (A0208, Beyotime, China) were used as secondary antibodies. For immunofluorescence experiments, Goat anti-mouse IgG and goat anti-rabbit IgG (Alexa Fluor® Plus 48, Alexa Fluor® Plus 488, 555647) purchased from Thermo Fisher Scientific were used as secondary antibodies. Palmitic acid and lactic acid were purchased from Sigma (USA), and MCT4 inhibitor VB124 was obtained from Selleck (USA).

Alveolar macrophages were identified by CD68 surface markers, and M1 or M2 macrophages were distinguished using iNOS and arginase-1 surface markers, respectively. Paraffin-embedded lung tissue sections (4 μm) of the TB patients were pretreated with ethylenediaminetetraacetic acid (EDTA) antigen-retrieval citrate buffer (C9999; Sigma Aldrich) at 120 °C for 4 min in a pressure boiler. The sections were incubated with anti-CD68 (sc-20,060, 1:100; Santa Cruz Biotechnology), anti-iNOS (sc-651, 1:100; Santa Cruz Biotechnology) and anti-arginase-1 (sc-20,150, 1:100; Santa Cruz Biotechnology) primary antibodies overnight at 4 °C. Immunoreactivity was detected using Alexa Fluor 488-conjugated goat anti-mouse IgG secondary antibody (A-11001, 1:200; Thermo) and Alexa Fluor 594-conjugated goat anti-rabbit IgG secondary antibody (A-11012, 1:200; Thermo). The slides were then mounted.