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Siva1 a01

Manufactured by Abnova
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SIVA1 (A01) is a laboratory equipment product offered by Abnova. It serves as a core functional device for specialized applications. The detailed technical specifications and intended use of this product are not available for presentation in an unbiased and factual manner within the constraints of this request.

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Lab products found in correlation

Pepstatin a, by Merck Group (2 mentions) Scrambled sirna, by Thermo Fisher Scientific (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) Mg132, by Merck Group (2 mentions) Bafilomycin a, by Merck Group (2 mentions) Anti beclin 1, by Cell Signaling Technology (2 mentions) Anti β actin clone ac 74, by Merck Group (2 mentions) Pcdna3 arf, by Addgene (2 mentions) Anti trip12 a301 814a, by Fortis Life Sciences (2 mentions) Anti mkrn1 a300 990a, by Fortis Life Sciences (2 mentions) Pbabe puro mcherry egfp lc3b, by Addgene (2 mentions) Hrp conjugated anti mouse igg, by Promega (2 mentions) Sirnas, by Integrated DNA Technologies (2 mentions) Anti lc3b, by Cell Signaling Technology (2 mentions)

2 protocols using siva1 a01

1

Molecular Mechanisms of Autophagy Regulation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
CMV driven HA-ubiquitin and pSP65SRα-CagA plasmids have been described previously30 (link), 31 (link). pBABE-puro mCherry-EGFP-LC3B and pcDNA3-ARF constructs were purchased from Addgene (Cambridge, MA)32 (link).
siRNAs against p14ARF and TRIP12 were synthesized by Integrated DNA Technologies (Coralville, Iowa) using the following sequences 5′-CAUGGUGCGCAGGUUCUUGGUGACC-3′ and 5′-CCAGGAGCAACAACUGAAAUCUGCA-3′, respectively. The negative control (scrambled) siRNA was purchased from Ambion (Grand Island, NY).
Following primary antibodies were used in this research: rabbit monoclonal anti-p14ARF (clone EPR17878, ab185620) from Abcam (Cambridge, MA), mouse monoclonal anti-β-actin (clone AC-74) from Sigma (St. Louis, MO), mouse monoclonal anti-GFP from Vanderbilt Monoclonal Antibody Core (Nashville, TN), rabbit polyclonal anti-beclin-1 (#3738), anti-LC3B (#2775) from Cell Signaling (Danvers, MA), rabbit polyclonal anti-TRIP12 (A301-814A) and anti-MKRN1 (A300-990A) from Bethyl Laboratories (Montgomery, TX), rabbit polyclonal SIVA1 (A01) from Abnova (Walnut, CA). The secondary antibodies used were HRP-conjugated anti-mouse IgG from Promega and anti-rabbit IgG from Cell Signaling.
Proteasomal inhibitor MG132 was purchased from EMD Millipore (Burlington, MA). Cycloheximide, bafilomycin A, pepstatin A and E-64d were all from Sigma.
Horvat A., Noto J.M., Ramatchandirin B., Zaika E., Palrasu M., Wei J., Schneider B.G., El-Rifai W., Peek RM J.r, & Zaika A.I. (2018). Helicobacter pylori pathogen regulates p14ARF tumor suppressor and autophagy in gastric epithelial cells. Oncogene, 37(37), 5054-5065.
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2

Molecular Mechanisms of Autophagy Regulation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
CMV driven HA-ubiquitin and pSP65SRα-CagA plasmids have been described previously30 (link), 31 (link). pBABE-puro mCherry-EGFP-LC3B and pcDNA3-ARF constructs were purchased from Addgene (Cambridge, MA)32 (link).
siRNAs against p14ARF and TRIP12 were synthesized by Integrated DNA Technologies (Coralville, Iowa) using the following sequences 5′-CAUGGUGCGCAGGUUCUUGGUGACC-3′ and 5′-CCAGGAGCAACAACUGAAAUCUGCA-3′, respectively. The negative control (scrambled) siRNA was purchased from Ambion (Grand Island, NY).
Following primary antibodies were used in this research: rabbit monoclonal anti-p14ARF (clone EPR17878, ab185620) from Abcam (Cambridge, MA), mouse monoclonal anti-β-actin (clone AC-74) from Sigma (St. Louis, MO), mouse monoclonal anti-GFP from Vanderbilt Monoclonal Antibody Core (Nashville, TN), rabbit polyclonal anti-beclin-1 (#3738), anti-LC3B (#2775) from Cell Signaling (Danvers, MA), rabbit polyclonal anti-TRIP12 (A301-814A) and anti-MKRN1 (A300-990A) from Bethyl Laboratories (Montgomery, TX), rabbit polyclonal SIVA1 (A01) from Abnova (Walnut, CA). The secondary antibodies used were HRP-conjugated anti-mouse IgG from Promega and anti-rabbit IgG from Cell Signaling.
Proteasomal inhibitor MG132 was purchased from EMD Millipore (Burlington, MA). Cycloheximide, bafilomycin A, pepstatin A and E-64d were all from Sigma.
Horvat A., Noto J.M., Ramatchandirin B., Zaika E., Palrasu M., Wei J., Schneider B.G., El-Rifai W., Peek RM J.r, & Zaika A.I. (2018). Helicobacter pylori pathogen regulates p14ARF tumor suppressor and autophagy in gastric epithelial cells. Oncogene, 37(37), 5054-5065.
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