Skbr 3 atcc no htb 30

Solvents for the synthesis and purification were purchased from Sigma-Aldrich. All cell culture reagents were purchased from Gibco® (Germany). Flasks and multiwell plates for in vitro studies were obtained from Nunc (Germany). Amine terminated PAMAM G4 dendrimer, docetaxel/paclitaxel, PBS (phosphate buffered saline), FBS (fetal bovine serum) and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich. Trypan blue was purchased from Molecular Probes (USA). Herceptin (trastuzumab) was obtained from Roche Poland. Human breast adenocarcinoma’s cell lines: HER-2 negative (MCF-7 ATCC no. HTB-22) and HER-2 positive (SKBR-3 ATCC no. HTB-30) were purchased from ATCC (USA).

Solvents for the synthesis and purification were purchased from Sigma-Aldrich (Poznan, Poland). All cell culture reagents were purchased from Gibco^® (Life Technologies Polska Sp. z o. o., Warsaw, Poland). Flasks and multiwell transparent and black plates for in vitro studies were obtained from Nunc (Life Technologies Polska Sp. z o. o., Warsaw, Poland). PAMAM G4-NH₂ dendrimer, docetaxel, paclitaxel, phosphate buffered saline (PBS), fetal bovine serum (FBS), propidium iodide (PI) and ribonuclease A deoxyribonuclease-free were purchased from Sigma-Aldrich (Poznan, Poland). Trypan blue was purchased from Molecular Probes ((Thermo Scientific™, Warsaw, Poland). Annexin V and 2′,7′-dichlorodihydrofluorescein (H2DCF-DA) were purchased from BD Biosciences (Warsaw, Poland). Caspase-Glo^® 3/7 Assay, Caspase-Glo^® 8 Assay and Caspase-Glo^® 9 Assay systems were purchased from Promega Corporation (Mannheim, Germany). Trastuzumab (Herceptin) was obtained from Roche Poland (Poznan, Poland). Human breast adenocarcinoma′s cell lines, including HER-2 positive (SKBR-3 ATCC no. HTB-30) and HER-2 negative (MCF-7 ATCC no. HTB-22) were purchased from ATCC (LGC Standards Sp. z o. o., Lomianki, Poland).

All chemical reagents were purchased from commercial suppliers. Solvents for the synthesis were purchased from Sigma-Aldrich (Poznan, Poland). All cell culture reagents were purchased from Gibco^® (Life Technologies Polska Sp. z o. o., Warsaw, Poland). Flasks and multiwell plates for in vitro studies were obtained from Nunc (Life Technologies Polska Sp. z o. o., Warsaw, Poland). Amine terminated PAMAM G4 dendrimer, doxorubicin hydrochloride, PBS (phosphate buffered saline), FBS (fetal bovine serum) and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich. Trypan blue was purchased from Molecular Probes (Thermo Scientific™, Warsaw, Poland). Herceptin (trastuzumab) was a gift from Roche Poland. Human breast adenocarcinoma’s cell lines: HER-2 negative (MCF-7 ATCC no. HTB-22) and HER-2 positive (SKBR-3 ATCC no. HTB-30) were purchased from ATCC (LGC Standards Sp. z o. o., Lomianki, Poland).