Spss statistics 19.0 program

The pharmacokinetic parameters were calculated through noncompartmental analysis based on the DAS3.2.8 pharmacokinetic program (invented by the Clinical Trial Center of Shanghai University of Traditional Chinese Medicine, Shanghai, China), including the area under the concentration-time curve from time zero to the last sampling time point (AUC_0–t), area under the concentration-time curve from time zero to infinity (AUC_0–∞), maximum plasma concentration of puerarin (C_max), time to maximum plasma concentration of puerarin (T_max), elimination half-life (T_1/2), mean residence time (MRT), total clearance (Cl), and the volume of distribution at terminal state (Vz).
The calculation formula of the bioavailability of puerarin is as follows:

Absolute bioavailability:(1)F%=AUC0–t,i.g.×Dosei.v.AUC0–t,i.v.×Dosei.g.×100%.

Relative bioavailability:(2)F%=AUC0–t,Pue−CCMsAUC0–t,puerarin×100%.

Data are presented as the mean ± SD. The differences were analyzed by t-test using IBM SPSS Statistics 19.0 program. P < 0.05 was considered statistically significant.