Rats were anesthetized using isoflurane (5% induction, 2–3% maintenance). Lidocaine (10% m/v) was used for local anesthesia. Animals were fixed in a robot stereotaxic frame (StereoDrive, Neurostar, Germany). The coordinates for the site of the injection were theoretically determined based on the Paxinos & Watson (2007) rat brain atlas [93 ] and checked through histological evaluation of 30 µm brain slices from dye-infused SERT+/+ rats. The total volume of 2 μL of either BDNF lentivirus particles (transcript variant IV under CMV promoter, NM_001270633.1) or pLenti-C-mGFP control lentivirus particles, was bilaterally infused into the ventral hippocampus according to the following coordinates: AP −5.60 mm, ML ±5.0 mm, DV −7.6 mm. After surgery, animals were placed in IVC cages (Sealsafe Plus GR900 green line, Tecniplast, Italy) until sacrifice.
Sealsafe plus gr900 green line
Manufactured by Tecniplast
Sourced in Italy
The Sealsafe Plus GR900 green line is a lab equipment product designed for animal housing. It provides a controlled environment for animal subjects. The product's core function is to create a secure and regulated space for animal research and experimentation.
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2 protocols using sealsafe plus gr900 green line
1
Lentiviral BDNF Delivery to Rat Ventral Hippocampus
2
Lentiviral BDNF Injection in Rat Hippocampus
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Rats were anesthetized using isoflurane (5% induction, 2-3% maintenance). Lidocaine (10% m/v) was used for local anesthesia. Animals were fixed in a robot stereotaxic frame (StereoDrive, Neurostar, Germany). The coordinates for the site of the injection were theoretically determined based on the Paxinos & Watson (2007) rat brain atlas and checked through histological evaluation of 30 µm brain slices from dye-infused SERT +/+ rats. The total volume of 2μL of either BDNF lentivirus particles (transcript variant IV under CMV promoter, NM_001270633.1) or pLenti-C-mGFP control lentivirus particles, was bilaterally infused into the ventral hippocampus according to the following coordinates: AP -5.60 mm, ML ±5.0 mm, DV -7.6 mm. After surgery, animals were placed in IVC cages (Sealsafe Plus GR900 green line, Tecniplast, Italy) until sacrifice.
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
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