Recombinant human ifnα

Manufactured by Miltenyi Biotec

Recombinant human IFNα is a laboratory product that serves as a source of the human interferon alpha protein. Interferon alpha is a type of cytokine that plays a role in the immune response.

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Lab products found in correlation

Complete rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Fetal calf serum (fcs), by Biowest (2 mentions) Streptavidin nanobeads, by BioLegend (1 mentions) Recombinant human m csf, by Miltenyi Biotec (1 mentions) Ld column, by Miltenyi Biotec (1 mentions) Cd14 magnetic beads, by Miltenyi Biotec (1 mentions) Recombinant human il 4, by Immunotools (1 mentions) Gm csf, by Immunotools (1 mentions) Magnetic bead, by Miltenyi Biotec (1 mentions)

2 protocols using recombinant human ifnα

Monocyte-derived Macrophages and Dendritic Cells

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Monocytes isolated using Percoll gradient or CD14-magnetic beads (Miltenyi Biotec) were cultured for 5 to 6 d in RPMI 1640 complete medium (Thermo Fisher Scientific) containing 10% fetal calf serum (Biowest), 50 U/mL penicillin, 50 μg/mL streptomycin, and 2 mM glutamine (all from Thermo Fisher Scientific). For generation of moDCs, monocytes were cultured in the presence of recombinant human IL-4 (500 U/mL) and GM-CSF (800 U/mL; both from Immunotools). For generation of moMacs, recombinant human M-CSF (50 ng/mL; Miltenyi Biotec) was used. To increase CD169 expression, cells were treated with recombinant human IFNα (1,000 U/mL; Miltenyi Biotec) during the last 2 d of culture. For enrichment of primary DCs, PBMCs were depleted from non-DC populations using biotinylated antibodies against CD3, CD14, CD19, CD56 (10 µg/mL; all produced and validated in-house), and CD16 (5 µg/mL; Biolegend) and streptavidin nanobeads (Biolegend) using an LD Column (Miltenyi).

Affandi A.J., Grabowska J., Olesek K., Lopez Venegas M., Barbaria A., Rodríguez E., Mulder P.P., Pijffers H.J., Ambrosini M., Kalay H., O’Toole T., Zwart E.S., Kazemier G., Nazmi K., Bikker F.J., Stöckl J., van den Eertwegh A.J., de Gruijl T.D., Storm G., van Kooyk Y, & den Haan J.M. (2020). Selective tumor antigen vaccine delivery to human CD169+ antigen-presenting cells using ganglioside-liposomes. Proceedings of the National Academy of Sciences of the United States of America, 117(44), 27528-27539.

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Isolation and Activation of Human CD14+ Monocytes

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CD14⁺ monocytes were isolated using magnetic beads (Miltenyi) on LS column according to manufacturer’s recommendation. Where indicated, monocytes were isolated using Percoll density gradient. Monocytes were cultured in RPMI 1640 complete medium (Thermo Fisher Scientific) containing 10% fetal calf serum (Biowest), 50 U/mL penicillin, 50 μg/mL streptomycin, and 2 mM glutamine (all from Thermo Fisher Scientific). Monocytes were then treated with recombinant human IFNα (Miltenyi Biotec) at indicated doses and time-points.

Affandi A.J., Olesek K., Grabowska J., Nijen Twilhaar M.K., Rodríguez E., Saris A., Zwart E.S., Nossent E.J., Kalay H., de Kok M., Kazemier G., Stöckl J., van den Eertwegh A.J., de Gruijl T.D., Garcia-Vallejo J.J., Storm G., van Kooyk Y, & den Haan J.M. (2021). CD169 Defines Activated CD14+ Monocytes With Enhanced CD8+ T Cell Activation Capacity. Frontiers in Immunology, 12, 697840.

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