For serum collection, mice were anesthetized with 0.1ml / 20 g body weight of Ketamine/xylazine cocktail (Ketamine, 20 mg / ml, xylazine, 2.5 mg/ ml), blood was withdraw by cardiac puncture, and was kept at room temperature for 2 – 3 hours. The blood was then centrifuged at 14000 rpm for 15 min at 4°C, serum was collected and stored at −80°C. Serum total IgG1and IgE levels were detected by ELISA using IgE and IgG1 ELISA kit from Biolegend. N. brasilensis excretory / secretory product (NES) was coated on 96 well ELISA plate overnight (2.5 μg/μl, 100 μl) and then incubated with serial dilutions of serum from N. brasiliensis inoculated mice or naive control mice and then processed using commercial ELISA kit.
Ige and igg1 elisa kit
Manufactured by BioLegend
The IgE and IgG1 ELISA kit is a laboratory equipment used for the quantitative measurement of IgE and IgG1 levels in biological samples. It is designed to provide accurate and reliable results through an enzyme-linked immunosorbent assay (ELISA) technique.
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2 protocols using ige and igg1 elisa kit
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Serum IgG1 and IgE ELISA Protocol
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Serum IgG1 and IgE ELISA Protocol
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For serum collection, mice were anesthetized with 0.1ml / 20 g body weight of Ketamine/xylazine cocktail (Ketamine, 20 mg / ml, xylazine, 2.5 mg/ ml), blood was withdraw by cardiac puncture, and was kept at room temperature for 2 – 3 hours. The blood was then centrifuged at 14000 rpm for 15 min at 4°C, serum was collected and stored at −80°C. Serum total IgG1and IgE levels were detected by ELISA using IgE and IgG1 ELISA kit from Biolegend. N. brasilensis excretory / secretory product (NES) was coated on 96 well ELISA plate overnight (2.5 μg/μl, 100 μl) and then incubated with serial dilutions of serum from N. brasiliensis inoculated mice or naive control mice and then processed using commercial ELISA kit.
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