Calbiosorb resin

All aqueous solutions were prepared using Millipore® DirectQ ultra-pure apyrogenic water. Bovine serum albumin (BSA), tris hydroxymethyl-amino-methane (Tris), sodium dodecylsulfate (SDS), p-nitrophenyl phosphate disodium salt (pNPP), dexamethasone, β-glycerophosphate, polyoxyethylene-9-lauryl ether (polidocanol) and glutaraldehyde (Grade I, specially purified for use as an electron microscopy fixative) were obtained from Sigma Chemical Co. (St. Louis, MO). Calbiosorb resin was from Merck Chemicals (Darmstadt, Germany), and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phospho-l-serine (DPPS) from Avanti Polar Lipids, Inc. (Alabaster, AL). α-MEM, fetal bovine serum, ascorbic acid, gentamicin and Fungizone were from Gibco-Life Technologies (Grand Island, NY). All reagents were analytical grade and used without further purification.

All aqueous solutions were made using Millipore DirectQ ultra pure apyrogenic water. Bovine serum albumin (BSA), tris hydroxymethyl-amino-methane (Tris), ATP, ADP, PP_i, sodium dodecylsulfate (SDS), dexamethasone, glucose 1-phosphate, glucose 6-phosphate, fructose 6-phosphate, β-glycerophosphate, polyoxyethylene-9-lauryl ether (polidocanol), α-naphthyl phosphate, Fast Blue RR, 2′,7′bis(carboxymethyl)-aminomethylfluorescein (CF), sucrose, glucose, HCl, MgCl₂, NaOH, NaCl, CaCl₂, KCl, NaHCO₃, KH₂PO₄, ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), were from Sigma Chemical Co. (St Louis, MO, USA), Calbiosorb resin (Merck Chemicals), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phospho-l-serine (DPPS) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were from Avanti Polar Lipids, Inc., ⁴⁵CaCl₂ (PerkinElmer, Inc.), Amicon Ultra 30 K device (30,000 NMWL), 75 cm² plastic culture flasks were from Corning (Cambridge, MA, USA). α-MEM, fetal bovine serum, ascorbic acid, gentamicin and fungizone were from Gibco-Life Technologies (Grand Island, NY, USA). Analytical grade reagents were used without further purification.