Bombesin

Manufactured by Bio-Techne

Sourced in United States, United Kingdom

Bombesin is a 14-amino acid neuropeptide that functions as a neuromodulator and neurotransmitter. It is found in the gastrointestinal tract and central nervous system. Bombesin can activate various receptor subtypes and is involved in regulating physiological processes such as gastrointestinal motility, secretion, and appetite.

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Lab products found in correlation

Yohimbine, by Bio-Techne (1 mentions) Norepinephrine ne, by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Rc 3095, by Merck Group (1 mentions) Bim23042, by Bio-Techne (1 mentions) Bapta am, by Merck Group (1 mentions) Wortmannin, by Cayman Chemical (1 mentions) Sdf 1α, by Thermo Fisher Scientific (1 mentions) Ym 254890, by Focus Biomolecules (1 mentions) Carbachol, by Thermo Fisher Scientific (1 mentions)

4 protocols using bombesin

Neuroreceptor Activation Assay

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The reagents used were as follows; Norepinephrine (NE) (Sigma Aldrich), Yohimbine, Bombesin (Tocris Bioscience), PBS, Insulin (Sigma corporation, St Louis, MO), 11-cis-retinal (National Eye Institute). Stock solutions of compounds were prepared according to manufacturers' recommendations. Before adding to cells, all stock solutions were diluted in 1% Hank's balanced salt solution (HBSS) or regular cell culture medium.

Wijayaratna D., Ratnayake K., Ubeysinghe S., Kankanamge D., Tennakoon M, & Karunarathne A. (2023). The spatial distribution of GPCR and Gβγ activity across a cell dictates PIP3 dynamics. Scientific Reports, 13, 2771.

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Investigating Bombesin-Mediated Signaling

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Bag-1 and bantag-1, BRS3 agonist and antagonist, respectively, were a kind gift from Dr Marc Reitman (Merck, Rahway, NJ USA). Bombesin, GRP, BIM 23042 were purchased from Tocris (Ellisville, MO, USA). RC-3095, TTX, CNQX, AP-5 and gabazine, BAPTA-AM were purchased from Sigma (St Louis, MO, USA).

Blais K., Sethi J, & Tabarean I.V. (2016). Gastrin-releasing peptide receptor mediates the excitation of preoptic GABAergic neurons by bombesin. Neuroscience letters, 633, 262-267.

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Cell Signaling Assay Protocols

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The reagents used were as follows: carbachol (Fisher Scientific), bombesin (Tocris Bioscience), SDF-1α (PeproTech), NE and isoproterenol (Sigma-Aldrich), YM-254890 (Focus Biomolecules), atropine, Ptx, U-50488, and wortmannin (Cayman Chemical), 11-cis-retinal (National Eye Institute). According to the manufacturer's instructions, all of the reagents were dissolved in appropriate solvents and diluted in 1% Hank's balanced salt solution supplemented with NaHCO₃, or a regular cell culture medium, before adding to cells.

Kankanamge D., Ubeysinghe S., Tennakoon M., Pantula P.D., Mitra K., Giri L, & Karunarathne A. (2021). Dissociation of the G protein βγ from the Gq–PLCβ complex partially attenuates PIP2 hydrolysis. The Journal of Biological Chemistry, 296, 100702.

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Preparation of Bioactive Compounds

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The reagents bombesin (Tocris Bioscience, Bristol, UK), 11-cis retinal (National Eye Institute, Bethesda, MD), and Tipifarnib and GGTI286 (Cayman Chemical, Ann Arbor, MI) were dissolved in appropriate solvents according to manufacturer’s instructions and diluted in 1% Hanks' Balanced Salt solution (HBSS) supplemented with NaHCO₃ or regular cell culture medium before being added to cells.

Tennakoon M., Senarath K., Kankanamge D., Chadee D.N, & Karunarathne A. (2021). A short C-terminal peptide in Gγ regulates Gβγ signaling efficacy. Molecular Biology of the Cell, 32(16), 1446-1458.

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