Amplified viral DNA, cDNA and total microbial DNA were used for shotgun library construction. DNA concentration was measured by Quant-iT PicoGreen dsDNA Assay kit (Thermo Fisher Scientific), and the fluorescence was detected by EnVision Multilabel Plate Reader (Waltham, MA, USA). Libraries were made using an Illumina Nextera XT Samples Prep kit (Illumina, San Diego, CA, USA), quantified using both Quant-iT PicoGreen dsDNA Assay kit and KAPA Library Quantification kit (Kapa Biosystems, Basel, Switzerland). The size distribution of the libraries was checked by 5300 Fragment Analyzer (Agilent, Santa Clara, CA, USA). Libraries were pooled for sequencing. The concentration of the pooled libraries was measured using Qubit (Invitrogen, Carlsbad, CA, USA), and the size distribution of the pooled libraries was checked by Agilent Technology 2100 Bioanalyzer using a High Sensitivity DNA chip (Agilent). Sequence was acquired using the Illumina Miseq (250 bp paired-end reads, Illumina) and HiSeq (150 bp paired-end reads, Illumina).
Nextera xt samples prep kit
Manufactured by Illumina
Sourced in United States
The Illumina Nextera XT Sample Prep kit is a library preparation solution for next-generation sequencing. It enables efficient conversion of DNA samples into sequencing-ready libraries. The kit utilizes a proprietary tagmentation process to simultaneously fragment and tag DNA samples, streamlining the library preparation workflow.
Automatically generated - may contain errors
Lab products found in correlation
Envision multilabel plate reader, by Thermo Fisher Scientific (2 mentions)
Quant it picogreen dsdna assay kit, by Roche (2 mentions)
Fragment analyzer 5300, by Agilent Technologies (2 mentions)
Agilent technology 2100 bioanalyzer, by Agilent Technologies (2 mentions)
Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (2 mentions)
Kapa library quantification kit, by Roche (2 mentions)
High sensitivity dna chip, by Agilent Technologies (2 mentions)
2 protocols using nextera xt samples prep kit
1
Shotgun Sequencing of Diverse DNA Samples
2
Shotgun Sequencing of Diverse DNA Samples
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Amplified viral DNA, cDNA and total microbial DNA were used for shotgun library construction. DNA concentration was measured by Quant-iT PicoGreen dsDNA Assay kit (Thermo Fisher Scientific), and the fluorescence was detected by EnVision Multilabel Plate Reader (Waltham, MA, USA). Libraries were made using an Illumina Nextera XT Samples Prep kit (Illumina, San Diego, CA, USA), quantified using both Quant-iT PicoGreen dsDNA Assay kit and KAPA Library Quantification kit (Kapa Biosystems, Basel, Switzerland). The size distribution of the libraries was checked by 5300 Fragment Analyzer (Agilent, Santa Clara, CA, USA). Libraries were pooled for sequencing. The concentration of the pooled libraries was measured using Qubit (Invitrogen, Carlsbad, CA, USA), and the size distribution of the pooled libraries was checked by Agilent Technology 2100 Bioanalyzer using a High Sensitivity DNA chip (Agilent). Sequence was acquired using the Illumina Miseq (250 bp paired-end reads, Illumina) and HiSeq (150 bp paired-end reads, Illumina).
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