4 nitrophenyl α d maltoheptaoside

All chemicals and solvents were at least HPLC-grade or higher. Water was purified using an Arium 611VF water purification system (Sartorius, Goettingen, Germany). Trypsin (from bovine pancreas, TPCK-treated, ≥ 10,000 BAEE U/mg protein), sodium hydroxide solution (50-52%), and anhydrous sodium acetate (≥ 99%) were obtained from Sigma-Aldrich (Steinheim, Germany). α-Amylase assay reagent (4-nitrophenyl α-d-maltoheptaoside (blocked), plus excess α-glucosidase and glucoamylase) and β-amylase assay reagent (4-nitrophenyl β-d-maltotrioside, plus excess thermostable β-glucosidase) were purchased from Megazyme (Bray, Ireland). d-(+)-glucose (≥ 99.5%), d-(-)-fructose (≥ 99%), sucrose (≥ 99.5%), maltose monohydrate (≥ 99%), maltotriose (≥ 90%), and maltoheptaose (≥ 70%) were obtained from Sigma-Aldrich. Maltotetraose (≥ 97%), maltopentaose (≥ 90%), maltohexaose (≥ 95%) and maltooctaose (≥ 80%) were purchased from Santa Cruz (Dallas, USA). Wheat flour type 550 was provided by Rosenmühle (Ergolding, Germany).

All chemicals and solvents were HPLC-grade or higher. Water was deionized and purified using an Arium 611VF water purification system (Sartorius, Goettingen, Germany). Trypsin (from bovine pancreas, TPCKtreated, ≥ 10,000 BAEE U/mg protein), sodium hydroxide solution (50-52%; eluent for IC) and anhydrous sodium acetate (≥ 99%) were from Sigma-Aldrich (Steinheim, Germany). α-Amylase assay reagent (4-nitrophenyl α-dmaltoheptaoside (blocked), plus excess α-glucosidase and glucoamylase) and β-amylase assay reagent (4-nitrophenyl β-d-maltotrioside, plus excess thermostable β-glucosidase) were purchased from Megazyme (Bray, Ireland). d-( +)-Glucose (≥ 99.5%), d-(-)-fructose (≥ 99%), sucrose (≥ 99.5%), maltose monohydrate (≥ 99%), maltotriose (≥ 90%) and maltoheptaose (≥ 70%) were from Sigma-Aldrich; maltotetraose (≥ 97%), maltopentaose (≥ 90%), maltohexaose (≥ 95%) and maltooctaose (≥ 80%) from Santa Cruz (Dallas, USA). Wheat flour type 550, according to the German flour classification system, was provided by Rosenmühle (Ergolding, Germany).