Matrimix

Manufactured by Nippi

Sourced in Japan

MatriMix is a laboratory mixing device designed for the homogenization of samples. It features a digital speed control and a timer function to ensure consistent mixing. The device's compact size makes it suitable for use in various laboratory settings.

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Lab products found in correlation

Balb cslc nu nu, by Japan SLC (1 mentions) Isoflurane inhalation solution, by Pfizer (1 mentions)

2 protocols using matrimix

Tumor Growth Suppression via SUV420H1 Modulation

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All animal experiments were performed with 6‐week‐old athymic nude mice (BALB/cSlc‐nu/nu: Japan SLC). For tumor injection, HCC cells of 1.0 × 10⁶ with SUV420H1 CRISPR/Cas9 KO plasmid and SUV420H1 HDR plasmid (CRISPR/Cas9 SUV420H1 KO) were suspended with 70 μL of growth medium and 200 μL of MatriMix (Nippi) following the manufacturer's protocol. HCC cells of 1.0 × 10⁶ with control CRISPR/Cas9 plasmid (CRISPR/Cas9 control) were similarly suspended. The solution of 200 μL containing HCC cells was then subcutaneously injected into the right lateral back of mice. The tumor size was measured every 7 days with calipers, and estimated tumor volumes were calculated with a formula:

({width}^{2} \times length) / 2 .

All mice were sacrificed at 28 days after the inoculation, and the size of resected tumors was recorded. The experiment was done in triplicate.

Kato H., Hayami S., Ueno M., Suzaki N., Nakamura M., Yoshimura T., Miyamoto A., Shigekawa Y., Okada K., Miyazawa M., Kitahata Y., Ehata S., Hamamoto R., Yamaue H, & Kawai M. (2023). Histone methyltransferase SUV420H1/KMT5B contributes to poor prognosis in hepatocellular carcinoma. Cancer Science, 115(2), 385-400.

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Allogeneic iPSC-CM Transplantation in SCID Rat Models

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After an acclimatization period of at least one week, the inguinal skin of 8-week-old male SCID rats (F344-II2rg/Rag2^em1lexa) was incised under isoflurane (Isoflurane Inhalation Solution, Pfizer Japan, Tokyo, Japan) inhalation anesthesia. Human iPSC-CMs at 1 × 10⁷ were suspended in MatriMix (511) with 10 µmol/L Y-27632 (Cat. #. 899001, Nippi, Tokyo, Japan) and injected into the inguinal subcutaneous adipose tissue. ^99mTcO₄⁻ SPECT/CT was performed 1, 6, and 10 weeks after cell injection.
After an acclimatization period of at least one week, 10-week-old male SCID rats were intubated and ventilated, and the left fourth intercostal space was opened under isoflurane inhalation anesthesia. A 7 mm-diameter aluminum rod cooled in liquid nitrogen for 1 min was placed once on the left ventricle to create cryoinjury, and 1 × 10⁷ human iPSC-CMs suspended in MatriMix (511) with 10 µmol/L Y-27632 (total volume 100 µl) were then injected into three locations around the injury. ^99mTcO₄⁻ SPECT/CT was performed 12 and 16 weeks after cell injection.

Saito Y., Nose N., Iida T., Akazawa K., Kanno T., Fujimoto Y., Sasaki T., Akehi M., Higuchi T., Akagi S., Yoshida M., Miyoshi T., Ito H, & Nakamura K. (2023). In vivo tracking transplanted cardiomyocytes derived from human induced pluripotent stem cells using nuclear medicine imaging. Frontiers in Cardiovascular Medicine, 10, 1261330.

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