Ix83 confocal laser scanning microscope

Treated cassava sections stained with coriphosphine-O were analysed under the IX83 Confocal Laser Scanning Microscope (Olympus, Japan). Samples were excited at 488 and 561 nm and emission was detected in 490–530 and 600–650 nm spectra, respectively. All pictures were taken with a magnification of 20x.
Confocal images of immunolabelled cassava sections treated with buffer (control) and with the NSPase product (dosed at 250 and 400 ppm) were taken using a IX83 Confocal Laser Scanning Microscope (Olympus, Japan). All samples were analysed with a 20x objective. Alexa-555 fluorescein-signal was excited at 561 nm (green) and detected in an emission spectrum between 570–620 nm, designated as red in the software. Cassava samples were excited at 405 nm (blue) and autofluorescence emission was observed within 429–470 nm, designated as green in the software.
The images were processed using the FV31S-SW software (Olympus, Japan).

The confocal microscope IX83 Confocal Laser Scanning Microscope (Olympus, Japan) equipped with the software FV31S-SW software (Olympus, Japan) was used for confocal imaging. The following excitation and emission wavelengths were used: Alexa-555 and Pontamine Fast Scarlet (ex 561 nm/em 570–620 nm), Calcofluor White and autofluorescence (ex 405 nm/em 430–470 nm). Laser intensity for Calcofluor White images was 0.03% (in contrast to 3% for autofluorescence) which ensures neglectable detection of autofluorescence signal. Optimal image settings were selected prior to the final images acquisition. Images were then adjusted for brightness and contrast using same settings for images that had to be compared.