The human HaCaT keratinocytes were purchased from Thermo Scientific (MA, USA) and SZ95 sebocytes were characterized as previously described46 (link). HaCaT cells and SZ95 sebocyteswere grown in Dulbecco’s Modified Eagle’s Medium (DMEM) or SebomedTM basal medium (Biochrom, Berlin, Germany), respectively, supplemented with 10% fetal bovine serum (Lonza, Basel, Switzerland), 100 U/mL penicillin, and 100 μl/mL streptomycin at 37 °C in a humidified incubator under 5% CO2.
Hacat keratinocytes
Manufactured by Thermo Fisher Scientific
Sourced in United States
HaCaT keratinocytes are a spontaneously immortalized human keratinocyte cell line derived from normal human skin. They are commonly used in cell culture research as a model for studying keratinocyte biology and skin-related applications.
Automatically generated - may contain errors
2 protocols using hacat keratinocytes
1
Cell Culture Protocol for HaCaT and SZ95 Cells
2
Culturing Immortalized Human DPCs and HaCaT Keratinocytes
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Immortalized human DPCs were a gift from Dr. Y. K. Sung (Department of Immunology and Hair Research Center, Kyungpook National University, Daegu, Korea) 30 .
HaCaT keratinocytes (an immortalized keratinocyte line) were purchased from Thermo Fisher Scientific. DPCs and HaCaT keratinocytes were cultured in DMEM (Hyclone) and supplemented with 10% FBS (Hyclone), 100 U/ml penicillin, and 100 µg/ml streptomycin (Gibco) at 37°C with 5% CO 2 .
HaCaT keratinocytes (an immortalized keratinocyte line) were purchased from Thermo Fisher Scientific. DPCs and HaCaT keratinocytes were cultured in DMEM (Hyclone) and supplemented with 10% FBS (Hyclone), 100 U/ml penicillin, and 100 µg/ml streptomycin (Gibco) at 37°C with 5% CO 2 .
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