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Inverted light fluorescent microscope model no ix81

Manufactured by Olympus
Sourced in Denmark

The Olympus Inverted Light/Fluorescent Microscope (Model No. IX81) is a versatile imaging system designed for a wide range of applications. It features a fully motorized setup with advanced optics and illumination systems to capture high-quality images and videos. The microscope is capable of both transmitted light and fluorescence imaging modes.

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2 protocols using inverted light fluorescent microscope model no ix81

1

Serum-deprived Cell Tube Formation

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Serum‐deprived cells (1.5 × 104 cells/well) were seeded on growth factor reduced Matrigel (50 μl) in 96 well plate and treated with or without TSA (0.33 μM). After 8 hrs, images were taken under 10× magnifications using an Olympus inverted light/fluorescent microscope (Model No. IX81). Tube length was measured using Image J software (National institute of Health, Bethesda, MD, USA) from 10 random fields and normalized to controls.
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2

Angiogenesis Assay with TGF-β1

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Serum-deprived cells (1.5 × 104 cells/well) were seeded on growth factor-reduced Matrigel (50 μl) in 96-well plate. Cells were treated with or without TGF-β1 (10 ng/ml) in the presence of EGM-2 media containing 2% FBS at 37°C, 5% CO2. After 8 hrs, images were taken under 4× and 10× magnifications by using an Olympus inverted light/fluorescent microscope (model no. IX81, Albertsturd, Denmark). Complete loop formation was counted from the entire well and normalized to controls. The investigator was blinded to the treatment groups.
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