Horseradish peroxidase hrp labeled goat anti rabbit igg

Mouse anti-HCV E1 monoclonal antibody (MAb) A4 (56 (link)) and mouse anti-YFV E MAb 2D12 (anti-E; ATCC CRL-1689) were produced in vitro by using a MiniPerm apparatus (Heraeus). Mouse anti-double-stranded RNA MAb (clone J2) was obtained from Scicons. Mouse anti-SARS-CoV-2 spike protein MAb were obtained from GeneTex. Polyclonal rabbit anti-SARS-CoV-2 nucleocapsid antibodies were from Novus. Cyanine 3-conjugated goat anti-mouse IgG and horseradish peroxidase (HRP)-labeled goat-anti-rabbit IgG antibodies were from Jackson Immunoresearch.

OTA (purity > 98%) was provided by Pribolab (Immunos, Singapore); Se-Y (contained Se 2000 mg/kg) was obtained from Angel Yeast (Beijing, China) and was dissolved in autoclaved saline eluent. Kits for analyzing ALT, AST, MDA, GSH-Px, SOD and T-AOC contents were provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Total tissue RNA extraction kit, cDNA synthesis kit and qRT-PCR kit were acquired from Vazyme (Nanjing, China). Anti-Caspase3 (1:900, Abcam, Tokyo, Japan), anti-Bax (1:900, Immunoway, Suzhou, China), anti-HO-1, anti-Bcl-2, anti-Nrf2 (1:800, Bioss, Beijing, China), anti-MnSOD (1:6000, Enzo, Farmingdale, NY, USA), anti-actin (1:10,000, Abcam, Tokyo, Japan) and the horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG (1:12,000, Jackson Immuno, PA, USA).