Discs containing SC samples were placed in 10 mL of 0.1% SDS and were sonicated for 2 min. Then, samples were centrifuged for 10 min at 4 °C, and 5500 rpm. Next, samples were dried and re-dissolved in 2 mL ultra-pure water. Protein concentration was determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) according to the manufacturer’s instruction in 96 well plastic plates. A 0.1% SDS was used as negative control. The protein measurement was performed at 595 nm wavelength on a Labsystems Multiskan MS spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA).
Labsystems multiskan ms spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in Finland
The Labsystems Multiskan MS is a spectrophotometer designed for absorbance measurements in microtiter plates. It features a built-in microplate reader and performs absorbance measurements across the visible and UV spectral range.
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Lab products found in correlation
3 protocols using labsystems multiskan ms spectrophotometer
1
SDS-Based Protein Extraction and Quantification
2
Serum CCL17 Measurement Protocol
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The blood sample was collected from the radial or femoral artery after sheath insertion before coronary angiography for serum CCL17 testing. The serum was isolated by centrifugation 3000rpm,10min and was frozen at -80℃ immediately until CCL17 measurement. Serum CCL17 level was tested via the Quantikine ELISA kit for human CCL17 (R&D Systems, Minneapolis, MN, USA) according to the manufacture's instruction.
The result was read through Labsystems Multiskan MS spectrophotometer (Thermo Labsystems Oy, Helsinki, Finland).
The result was read through Labsystems Multiskan MS spectrophotometer (Thermo Labsystems Oy, Helsinki, Finland).
3
Serum CCL17 Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The blood sample was collected from the radial or femoral artery after sheath insertion before coronary angiography for serum CCL17 testing. The serum was isolated by centrifugation 3000rpm,10min and was frozen at -80℃ immediately until CCL17 measurement. Serum CCL17 level was tested via the Quantikine ELISA kit for human CCL17 (R&D Systems, Minneapolis, MN, USA) according to the manufacture's instruction. The result was read through Labsystems Multiskan MS spectrophotometer (Thermo Labsystems Oy, Helsinki, Finland).
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