Rabbit anti sirt1

Manufactured by Proteintech

Sourced in China, United States

Rabbit anti-SIRT1 is a primary antibody that specifically recognizes the SIRT1 protein, a member of the sirtuin family of NAD+-dependent protein deacetylases. This antibody can be used to detect and study the expression and localization of SIRT1 in various biological samples.

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Lab products found in correlation

Rabbit anti phospho ampk thr172 antibody, by Cell Signaling Technology (1 mentions) Rabbit anti α sma antibody, by Proteintech (1 mentions) Rabbit anti β actin antibody, by Proteintech (1 mentions) Rabbit anti nox4 antibody, by Proteintech (1 mentions) Rabbit anti phospho akt ser473 antibody, by Cell Signaling Technology (1 mentions) Rabbit anti ampk antibody, by Proteintech (1 mentions) Rabbit anti nrf2 antibody, by Proteintech (1 mentions) Quantity one software, by Bio-Rad (1 mentions) Goat anti runx2, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

SIRT1 (31 citations) Anti-SIRT1 (13 citations) Anti-TM (2 citations) Polyclonal rabbit anti‐SIRT1 (2 citations)

2 protocols using rabbit anti sirt1

Liver Western Blot Analysis

Cited 1 time

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Two livers from each group were combined into one sample and total protein was extracted for western blot analysis (one sample mixed with liver tissues from two fish, n = 8).
Western blotting was performed as described previously [83 (link)]. The antibodies used were as follows: rabbit anti-β-actin antibody (1:2000, Proteintech, Wuhan, China), rabbit anti-α-SMA antibody (1:1000, Proteintech, Wuhan, China), rabbit anti-SIRT1 (1:1500, Proteintech, Wuhan, China), rabbit anti-NOX4 antibody (1:1000, Proteintech, Wuhan, China), rabbit anti-NRF2 antibody (1:1500, Proteintech, Wuhan, China), rabbit anti-phospho-AKT (Ser473) antibody (1:2000, Cell Signaling), rabbit anti-phospho-AMPK (Thr172) antibody (1:1000, Cell Signaling), rabbit anti-AMPK antibody (1:1000, Proteintech, Wuhan, China). Protein expression was normalized to that of β-actin.

Zou Y., Chen Z., Sun C., Yang D., Zhou Z., Peng X., Zheng L, & Tang C. (2021). Exercise Intervention Mitigates Pathological Liver Changes in NAFLD Zebrafish by Activating SIRT1/AMPK/NRF2 Signaling. International Journal of Molecular Sciences, 22(20), 10940.

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Western Blot Analysis of Sirt1, Runx2

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All samples were collected, and the Western blot was conducted as previously described [29] . The primary antibodies used were rabbit anti-Sirt1 (1:2000; Proteintech, Rosemont,USA), goat anti-Runx2 (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and mouse anti-GAPDH (1:10,000; Kangcheng, Shanghai, China). The protein bands were quantitatively analyzed using an image analysis system (QuantityOne software; Bio-Rad, Hercules, CA, USA).

Hong W., Wei Z., Qiu Z., Li Z., Fu C., Ye Z., & xu x. (2020). Atorvastatin Promotes Bone Formation in Aged apoE–/– Mice through the Sirt1–Runx2 Axis.

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