Approximately 4 x 103 cells (per well) with stable COL3A1 overexpression or downregulation were added onto 96-well plates and maintained for 4 h in a culture medium with 10% CCK-8 solution (Beyotime, Beijing, China) at 37 °C, after which their OD450 values were detected using a spectrophotometer (Fisherbrand™ accuSkan™ GO UV/Vis, Thermo).
Accuskan go uv vis
Manufactured by Thermo Fisher Scientific
Sourced in China
The AccuSkan™ GO UV/Vis is a compact, high-performance spectrophotometer designed for accurate and reliable absorbance measurements in the ultraviolet and visible light spectrum. It features a wavelength range of 190 to 1100 nanometers and can be used for a variety of laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using accuskan go uv vis
1
Cell Viability Assay with COL3A1 Modulation
2
Cell Growth Quantification Assay
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Cells were placed in 96-well plates with 4,000 cells in each well. For cell growth assessment, cells were cultured with 10% (v/v) CCK-8 solution (Beyotime, Beijing, China) for 4 hours at 37 °C. Then, the OD values (450 nm) were detected with a Spectrophotometer (Fisherbrand™ accuSkan™ GO UV/Vis, Thermo).
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