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Beckman 2

Manufactured by Beckman Coulter
Sourced in United States

The Beckman II is a laboratory centrifuge designed for general-purpose applications. It provides reliable and consistent performance for a variety of sample separation and preparation needs. The Beckman II is a compact and durable instrument built to deliver efficient and reproducible results.

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2 protocols using beckman 2

1

Plasma Biomarker Quantification Protocol

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Plasma glucose was assayed by the glucose oxidase method (Beckman II, Glucose Analyser, Fullerton CA); HbAlc was determined by high-performance liquid chromatography (DIAMAT, Bio-Rad, Richmond, CA, USA); plasma triglycerides and total cholesterol were determined enzymatically (Boehringer Mannheim, Germany). High-density lipoprotein (HDL)-cholesterol was measured by precipitation with heparin and MgCl2 on whole plasma, whereas low-density lipoprotein (LDL)-cholesterol was calculated by Friedewald’s formula.
THPO levels in plasma were quantified using a specific ELISA kit (R&D Systems) following the manufacturer’s instructions. The lower detection limit of the assay was 7.45 pg/mL.
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2

Tissue-Plasma Glucose Dynamics Monitoring

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The time delay between plasma and tissue during plasma glucose concentration increase (BT↑) was measured as time between maximum glucose concentration increase rates in plasma and tissue. Maximum glucose concentration increase in tissue was measured by CGMS. Similarly, the time delay at glucose concentration decrease (TB↓) was measured as the difference in CGMS and Beckman values at maximum decrease rates in tissue (measured by CGMS) and plasma glucose decrease rate. Both quantities are well defined from the mathematical point of view. BT↑ is defined as the time difference between inflex points of plasma and tissue glucose concentration when the glucose concentration increases. TB↓ is defined in the same manner when the glucose concentration in venous plasma decreases.
Capillary plasma glucose concentration was measured by the glucometer-strip (glucoseoxidase) system Calla and on the analyser Beckman II using glucoseoxidase enzyme. The best correlation between Beckman analyser and system Calla proved the accuracy of plasma glucose measurement and correct CGMS calibration using glucometer Calla.
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