The CCK-8 cell count kit (Solarbio, China) was used to evaluate the growth rate of bESCs. In brief, 3 × 103 cells were passaged onto MC-MEFs or MT-MEFs in a 48-well plate. The MC-MEFs or MT-MEFs in CTFR medium were used as parallel blank controls. Then, 20 μl of CCK-8 reagent was added into each well and incubated at 37 °C and 5% CO2 for 3 h. The absorbance was measured at 450 nm every 24 h for 3 days. The growth rate was calculated using the ratio of OD value at D (n)/D (n − 1).
Cck 8 cell count kit
Manufactured by Solarbio
Sourced in China
The CCK-8 cell count kit is a colorimetric assay for the determination of the number of viable cells in proliferation or cytotoxicity experiments. The kit utilizes the highly water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in living cells to produce a yellow-colored formazan dye. The amount of the formazan dye generated is directly proportional to the number of living cells.
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2 protocols using cck 8 cell count kit
1
Cell Growth Assay with CCK-8
2
Stem Cell Growth Assay Using CCK-8
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The CCK-8 cell count kit (CA1210, Solarbio, Beijing, China) was used to evaluate the growth rate of stem cells. In brief, 3 × 10 3 stem cells were passaged onto MC-MEFs or MT-MEFs in a 48-well plate. The stem cell free MC-MEFs or MT-MEFs in CTFR medium were used as parallel blank controls. Then, 20 µl of CCK-8 reagent was added into each testing well and incubated at 37°C and 5% CO 2 for 3 h. The absorbance was measured at 450 nm every 24 hours for 3 days. The growth rate was calculated using the ratio of OD value at D (n)/D (n-1).
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