The 76nt random oligonucleotide library, 5'-ATC CAG AGT GAC GCA GCA-40nt-TGG ACA CGG TGG CTT AGT-3' FITC labelled upstream primer 5'FITC-ATC CAG AGT GAC GCA GCA, and Biotin labelled downstream primer 5'Biotin-ACT AAG CCA CCG TGT CCA-3' were synthesised by Huada Biotech (Beijing, China) Co., Ltd. U2-OS cells, HOS cells, and HT-1080 cells were purchased from Boshide Bioengineering Co., Ltd. McCoy's 5A medium and high-sugar DMEM medium were all purchased from Boshide Bioengineering Co., Ltd (Wuhan, China). Yeast tRNA (Fluka Analytical, USA), heat-inactivated foetal bovine serum (FBS Invitrogen, USA), Streptavidin Sepharose ® HP (GE healthcare, USA), RPMI-1640 medium (GIBCO), 2x Taq PCR Master Mix, 50 bp DNA Ladder (Tiangen, Japan), and an agarose gel recovery kit (Dingguo, China) were also used.
Agarose gel recovery kit
Manufactured by Dingguo
Sourced in Japan
The Agarose Gel Recovery Kit is a laboratory equipment designed to extract and purify DNA fragments from agarose gel. It provides a simple and efficient method to recover DNA samples after electrophoresis.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using agarose gel recovery kit
1
Oligo Library Screening in Cell Lines
2
Screening of Oligonucleotide Libraries in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The 76nt random oligonucleotide library, 5'-ATC CAG AGT GAC GCA GCA-40nt-TGG ACA CGG TGG CTT AGT-3',FITC labelled upstream primer 5'FITC-ATC CAG AGT GAC GCA GCA, and Biotin labelled downstream primer 5'Biotin-ACT AAG CCA CCG TGT CCA-3' were synthesised by Huada Biotech (Beijing, China) Co., Ltd. U2-OS cells, HOS cells, and HT-1080 cells were purchased from Boshide Bioengineering Co., Ltd. McCoy's 5A medium and high-sugar DMEM medium were all purchased from Boshide Bioengineering Co., Ltd (Wuhan, China). Yeast tRNA (Fluka Analytical, USA), heat-inactivated foetal bovine serum (FBS Invitrogen, USA), Streptavidin Sepharose® HP (GE healthcare, USA), RPMI-1640 medium (GIBCO), 2x Taq PCR Master Mix, 50 bp DNA Ladder (Tiangen, Japan), and an agarose gel recovery kit (Dingguo, China) were also used.
3
Screening of Oligonucleotide Libraries in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 76nt random oligonucleotide library, 5'-ATC CAG AGT GAC GCA GCA-40nt-TGG ACA CGG TGG CTT AGT-3',FITC labelled upstream primer 5'FITC-ATC CAG AGT GAC GCA GCA, and Biotin labelled downstream primer 5'Biotin-ACT AAG CCA CCG TGT CCA-3' were synthesised by Huada Biotech (Beijing, China) Co., Ltd. U2-OS cells, HOS cells, and HT-1080 cells were purchased from Boshide Bioengineering Co., Ltd. McCoy's 5A medium and high-sugar DMEM medium were all purchased from Boshide Bioengineering Co., Ltd (Wuhan, China). Yeast tRNA (Fluka Analytical, USA), heat-inactivated foetal bovine serum (FBS Invitrogen, USA), Streptavidin Sepharose® HP (GE healthcare, USA), RPMI-1640 medium (GIBCO), 2x Taq PCR Master Mix, 50 bp DNA Ladder (Tiangen, Japan), and an agarose gel recovery kit (Dingguo, China) were also used.
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