1 2 dimyristoyl sn glycero 3 phospho 10 rac glycerol

HPLC grade chloroform, methanol and acetonitrile were obtained from Fisher Scientific Ltd. (Loughborough, UK). Lipid internal standards 1,2-dimyristoyl-sn-glycero-3-phosphate (dMPA), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (dMPC), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (dMPE), 1,2-dimyristoyl-sn-glycero-3-phospho-(10-rac-glycerol) (dMPG), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylinositol (dPPI) and 1-nonadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Milli-Q water (Synergy, Millipore Corporation, Billerica, MA, USA) was produced when ultrapure water was necessary. All other reagents were purchased from major commercial sources.

Reagents and solvents were purchased from commercial vendors and used without further purification. Rink amide MBHA resin, 2‐chlorotrityl resin, and Fmoc‐protected amino acids were purchased from Merck Millipore (Billerica, MA, USA). Trifluoroacetic acid (TFA) and N,N‐dimethylformamide (DMF, >99.9%, peptide synthesis grade) were purchased from Acros Organics (Fair Lawn, NJ, USA). Acetonitrile (ACN, HPLC grade), propidium iodide (PI), and FM4‐64 were purchased from Thermo Fisher Scientific (Waltham, MA, USA). N,N’‐Diisopropylcarbodiimide (DIC) was purchased from Chem‐Impex (Wood Dale, IL, USA). 1‐Palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphoethanolamine (POPE), 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine (POPC), 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phospho‐(1’‐rac‐glycerol) (POPG), 2‐dimyristoyl‐sn‐glycero‐3‐phosphocholine (DMPC), 1,2‐dimyristoyl‐sn‐glycero‐3‐phospho‐(10‐rac‐glycerol) (DMPG), and 1,2‐dimyristoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐[methoxy(polyethylene glycol)‐2000] (DMPE‐PEG) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Mueller‐Hinton broth 2 (MHB2), bovine serum albumin (BSA), thioflavin T (Th T), thioflavin S (Th S), acetic acid, poly‐L‐lysine solution, and human liver S9 were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Other chemical reagents and solvents were purchased from TCI (Tokyo, Japan) and Alfa Aesar (Haverhill, MA, USA).

Phospholipid internal standards 1,2-dimyristoyl-sn-glycero-3-phosphocholine (dMPC), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (dMPE), 1,2-dimyristoyl-sn-glycero-3-phospho-(10-rac-)glycerol (dMPG), 1,2-dimyristoyl-sn-glycero-3-phospho-L-serine (dMPS), tetramyristoylcardiolipin (TMCL), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylinositol (dPPI), N-palmitoyl-D-erythro-sphingosylphosphorylcholine (NPSM), 1-nonadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC) were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Chloroform, methanol and acetonitrile were purchased from Fisher scientific (Leicestershire, UK); all the solvents were of high performance liquid chromatography (HPLC) grade and were used without further purification. All the other reagents and chemicals used were of the highest grade of purity commercially available. The water was of Milli-Q purity (Synergy1, Millipore Corporation, Billerica, MA).

High-performance liquid chromatography (HPLC)-grade dichloromethane (CH₂Cl₂), absolute ethanol 96% (CH₃CH₂OH), and methanol (CH₃OH) were obtained from Fisher Scientific Ltd. (Loughborough, UK). Milli-Q purified water (Synergy1, Millipore Corporation, Billerica, MA, USA) was used in all experiments. The 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS^●+) was purchased from Fluka (Buchs, Switzerland) and the α,α-diphenyl-β-pic-rylhydrazyl radical (DPPH^●) from Aldrich (Milwaukee, WI, USA). All the remaining reagents were obtained from primary commercial sources. The lipid internal standards used in the lipidome analyses were acquired from Avanti Polar Lipids, Inc. (Alabaster, AL, USA): 1,2-dimyristoyl-sn-glycero-3-phosphate (dMPA), 1,2-dimyristoyl-sn-glycero-3-phospho-(10-rac-glycerol) (dMPG), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (dMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphatidylinositol (dPPI), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (dMPE), 1,2-dimyristoyl-sn-glycero-3-phosphatidylserine (dMPS), 1-nonadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC), N-heptadecanoyl-D-erythro-sphingosine (Cer), and 1′,3′-bis-[1-2-di-tetradecanoyl-sn-glycero-3-phospho]-sn-glycerol (CL), and N-heptadecanoyl-D-erythro-sphingosylphosphorylcholine (SM).