Cellometer auto t4 bright field cell counter

Manufactured by Revvity

Sourced in United States

The Cellometer Auto T4 Bright Field Cell Counter is a compact, automated cell counting instrument designed to provide accurate and consistent cell counts. It utilizes bright-field microscopy technology to capture images of cells and analyzes them to determine the total cell count and viability.

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Lab products found in correlation

Cell death detection elisa kit, by Roche (1 mentions) Synergy h1 hybrid multi mode microplate reader, by Agilent Technologies (1 mentions) Trypan blue solution, by Thermo Fisher Scientific (1 mentions) Trypan blue, by Thermo Fisher Scientific (1 mentions) Elisa quantitation kit, by Fortis Life Sciences (1 mentions) Elecsys 2010, by Roche (1 mentions) Dispase, by Roche (1 mentions)

Close spelling variants of this product

Cellometer (154 citations) Cellometer Auto T4 (152 citations) Cellometer Auto T4 cell counter (34 citations) Cell counter (19 citations) Cellometer cell counter (9 citations) T4 Cellometer (5 citations) Cellometer Auto T4 counter (3 citations) Cellometer Bright Field cell counter (2 citations) T4 cell counter (2 citations)

9 protocols using cellometer auto t4 bright field cell counter

Growth of Phaeodactylum tricornutum

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P. tricornutum UTEX646 (Pt4) was grown in F/2 medium^{40 (link)} at 20 °C under white fluorescent lights’, constant illumination (60 µmol photons m⁻² s⁻¹) and agitated at 70 rpm. For the cell growth experiment samples were collected during the growth curve from day 0 to the late exponential phase (12–13 d). Cell concentration was measured by triplicate cell counts using a Cellometer Auto T4 Bright Field Cell Counter (Nexcelom bioscience).

Pudney A., Gandini C., Economou C.K., Smith R., Goddard P., Napier J.A., Spicer A, & Sayanova O. (2019). Multifunctionalizing the marine diatom Phaeodactylum tricornutum for sustainable co-production of omega-3 long chain polyunsaturated fatty acids and recombinant phytase. Scientific Reports, 9, 11444.

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Spheroid Growth Characterization Protocol

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To better characterize the 3D culture model, we quantified the cells that form the spheroids at different time points (determinations at every 12 h). At each time point, the spheroids were removed from the agarose microwells and dissociated using trypsin. Isolated cells were counted using a Cellometer® Auto T4 Bright Field Cell Counter (Nexcelom Bioscience, Lawrence, MA). Triplicates were performed for each time point. We have thus drawn a spheroid growth curve to identify the exponential, stationary growth, and decline phases.

Monteleone-Cassiano A.C., Dernowsek J.A., Mascarenhas R.S., Assis A.F., Pitol D., Santos Moreira N.C., Sakamoto-Hojo E.T., Issa J.P., Donadi E.A, & Passos G.A. (2022). The absence of the autoimmune regulator gene (AIRE) impairs the three-dimensional structure of medullary thymic epithelial cell spheroids. BMC Molecular and Cell Biology, 23, 15.

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Cell Proliferation and Apoptosis Assay

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Cell number was measured using trypan blue exclusion on a cell counter device Cellometer Auto T4 Bright Field Cell Counter (Nexcelom Bioscience LLC, Lawrence, MA, USA). Cell viability/proliferation was measured by counting the cell numbers in using Crystal Violet staining followed by the measurement of absorbance at 600 nm using SOFTmaxPRO. Apoptosis was assayed using cell-death detection ELISA kits (Roche Diagnostic Corporation, Indianapolis, IN, USA).

Das A., Dhar K., Maity G., Sarkar S., Ghosh A., Haque I., Dhar G., Banerjee S, & Banerjee S.K. (2017). Deficiency of CCN5/WISP-2-Driven Program in breast cancer Promotes Cancer Epithelial cells to mesenchymal stem cells and Breast Cancer growth. Scientific Reports, 7, 1220.

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Cell Adhesion Assay with Collagen

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Ninety-six-well plates coated with 10 μg/cm² collagen (Sigma-Aldrich, catalog no. 8919) were washed with PBS and incubated for 1 hour with DMEM + 0.5% BSA at 37°C. Cells were detached with 10 mmol/L EDTA in PBS for 10 minutes at 37°C, washed twice with DMEM, counted using a Cellometer Auto T4 Bright Field Cell Counter (Nexcelom Bioscience), and diluted to a density of 1 × 10⁵ cells/mL in DMEM + 0.1% BSA. A total of 10,000 cells were added per well and incubated for 1 hour at 37°C. Loosely attached cells were removed by vigorous shaking of the plate for 10 seconds, and washing with DMEM + 0.1% BSA. Adherent cells were fixed with 4% paraformaldehyde for 10 minutes, washed, and stained for 10 minutes with 5 mg/mL crystal violet in 2% ethanol. Plates were washed once with water and then dried overnight. Crystal violet stain was solubilized with 200 μL 2% SDS/well for 30 minutes and diluted 1:4 with water. Absorption was measured at 550 nm using a Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek).

Popow O., Paulo J.A., Tatham M.H., Volk M.S., Rojas-Fernandez A., Loyer N., Newton I.P., Januschke J., Haigis K.M, & Näthke I. (2019). Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and β-Catenin-Independent Targets by Proteomics. Molecular cancer research : MCR, 17(9), 1828-1841.

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Quantifying VX2 Cell Concentration in CSF Filtration

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VX2 cell concentration was quantified after every full CSF volume cycle (150 mL) processed across the filters. For filtration studies without MTX infusion, total cell concentration was calculated with the samples using a 1:1 dilution with Acridine Orange on the Nexcelom Cellometer Auto T4 Bright Field Cell Counter. For filtration studies with MTX infusion, Trypan Blue Solution (0.4%; Thermo Scientific) was used to assess live and dead cell concentrations using a hemocytometer. All counts in which no cells were observed were reported as a concentration of 1 × 10³ cells/mL, the approximate limit of detection of the Nexcelom cell counter and hemocytometer.

Ejikeme T., de Castro G.C., Ripple K., Chen Y., Giamberardino C., Bartuska A., Smilnak G., Marius C., Boua J.V., Chongsathidkiet P., Hodges S., Pagadala P., Verbick L.Z., McCabe A.R, & Lad S.P. (2020). Evaluation of neurapheresis therapy in vitro: a novel approach for the treatment of leptomeningeal metastases. Neuro-oncology Advances, 2(1), vdaa052.

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Quantifying HCEC Viability in Wnt3a and Kallistatin

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HCEC (ATCC, no. PCS700010) were cultured in Corneal Epithelial Cell Basal Medium (ATCC, no. PCS700030) in a 24-well plate. After incubation with 20% L cell-conditioned medium (LCM) or Wnt3a-conditioned medium (WCM) and different concentrations of human kallistatin protein, the cell suspension was stained with trypan blue (Thermo Fisher). Viable cells were counted using Cellometer Auto T4 Bright Field Cell Counter (Nexcelom, Lawrence, MA).

Liang W., Huang L., Ma X., Dong L., Cheng R., Dehdarani M., Karamichos D, & Ma J.X. (2022). Pathogenic Role of Diabetes-Induced Overexpression of Kallistatin in Corneal Wound Healing Deficiency Through Inhibition of Canonical Wnt Signaling. Diabetes, 71(4), 747-761.

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Quantification of Albumin Secretion

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At the endpoint of the differentiation process, the supernatant of cultured cells was collected. Albumin secretion in the supernatant was measured with a human albumin enzyme-linked immunosorbent assay (ELISA) quantitation kit (Bethyl, Montgomery, TX, USA) according to the manufacturer’s instructions. Cells were trypsinized and counted with Cellometer Auto T4 Bright Field Cell Counter (Nexcelom Bioscience, Lawrence, MA, USA). The albumin secretion was normalized to total cell numbers.

Du C., Feng Y., Qiu D., Xu Y., Pang M., Cai N., Xiang A.P, & Zhang Q. (2018). Highly efficient and expedited hepatic differentiation from human pluripotent stem cells by pure small-molecule cocktails. Stem Cell Research & Therapy, 9, 58.

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Quantification of Secreted AFP

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The human alpha fetoprotein (AFP) content in the supernatant was determined with electrochemiluminescence immunoassay (Elecsys 2010, Roche Diagnostics, Basel, Switzerland) according to the manufacture’s protocol. Cells were trypsinized and counted with Cellometer Auto T4 Bright Field Cell Counter (Nexcelom Bioscience). The AFP secretion was normalized to total cell numbers.

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Dissociation of AIRE-Expressing Spheroids

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AIRE^WT and AIRE^−/− spheroids were cultured and after 12 h of adhesion the spheroids were incubated with dispase (Roche Diagnostics, Basel, Switzerland) for a maximum of 30 min to release cells as monolayers. Every 5 min released monolayers were carefully washed with PBS twice and subject to mechanical stress by pipetting with 1 mL pipettes, and the cells were counted using the Cellometer® Auto T4 Bright Field Cell Counter (Nexcelom Bioscience, Lawrence, MA). Triplicates were performed for each time point.

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