Sum ss18v

Manufactured by Nest Group

Sourced in United States

The SUM SS18V is a laboratory equipment product. It is a precision scale designed for weighing small samples with a maximum capacity of 18 kg and a readability of 0.1 g.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using sum ss18v

Fractionation of Peptides by SCX Chromatography

Check if the same lab product or an alternative is used in the 5 most similar protocols

For SCX chromatography, three aliquots (10 µl each) from the pool were depleted and separately digested. The digests were combined before fractionation. Mainly, a step-wise gradient of potassium chloride: 20, 40, 60, 100, 500 mM and 1 M KCl in 10 mM potassium phosphate, pH 2.8 (or 0.01% phosphoric acid) containing 20% acetonitrile was used to fractionate the peptides.
According to the instructions from the manufacturer, salt was removed from the samples by silica C18 ultra-microspin columns (SUM SS18V, 3–30 mg capacity, The Nest Group Inc., South Borough, MA, USA). After elution with 50% acetonitrile/0.1% TFA, the fractions were dried in a centrifugal evaporator and re-suspended in 0.1% formic acid prior to analysis by LC-MS/MS.

Pla I., Sanchez A., Pors S.E., Pawlowski K., Appelqvist R., Sahlin K.B., Poulsen L.L., Marko-Varga G., Andersen C.Y, & Malm J. (2020). Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation. Human Reproduction (Oxford, England), 36(3), 756-770.

+ Open protocol

+ Expand

Quantitative Proteomics Using RIME and Dimethyl-Labelling

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

RIME and stable isotope dimethyl-labelling were performed, as previously described^{17 (link),60 (link),61 (link)}. Briefly, cells were labelled with the medium and light isotope reagent. Labelled samples were pooled at an approximate 1:1 ratio, dried down and fractionated using 12 cm IPG strip pH 3-10^{62 (link)}. RIME and dimethyl-label fractions were desalted (SUM SS18V, The Nest Group Inc) and run through LC-MS/MS using LTQ Velos Orbitrap MS. Raw data for RIME and dimethyl-labelling were analysed using MaxQuant 1.5.1.0^{62 (link),63 (link)}. Statistical analysis was performed using Perseus software (version 1.6.1.3)^{64 (link)}. Raw data have been deposited to the ProteomeXchange Consortium via PRIDE partner repository with the dataset identifier PXD004648 (MCF-7) and PXD004807 (SUM44).

Montaudon E., Nikitorowicz-Buniak J., Sourd L., Morisset L., El Botty R., Huguet L., Dahmani A., Painsec P., Nemati F., Vacher S., Chemlali W., Masliah-Planchon J., Château-Joubert S., Rega C., Leal M.F., Simigdala N., Pancholi S., Ribas R., Nicolas A., Meseure D., Vincent-Salomon A., Reyes C., Rapinat A., Gentien D., Larcher T., Bohec M., Baulande S., Bernard V., Decaudin D., Coussy F., Le Romancer M., Dutertre G., Tariq Z., Cottu P., Driouch K., Bièche I., Martin L.A, & Marangoni E. (2020). PLK1 inhibition exhibits strong anti-tumoral activity in CCND1-driven breast cancer metastases with acquired palbociclib resistance. Nature Communications, 11, 4053.

+ Open protocol

+ Expand

Quantitative Proteomics Analysis of Breast Cancer

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

RIME^{22 (link)} and stable isotope dimethyl labeling^{21 (link)} were performed, as previously described. The wt-SUM44 and SUM44-LTED were labeled with the medium and light isotope reagent, respectively. Labeled samples were pooled at an approximate 1:1 ratio, dried down and fractionated using 12 cm IPG strip pH 3–10, as previously described^{46 (link)}. RIME and dimethyl label fractions were desalted (SUM SS18V, The Nest Group Inc) and run through LC-MS/MS using LTQ Velos Orbitrap MS. The data acquisition mode was set, as previously described^{46 (link)}. Raw data for RIME and dimethyl labeling were analyzed using MaxQuant 1.5.1.0^{46 (link),47 (link)}. Search parameters were as previously described^{46 (link)}. All proteomics data are deposited within the PRIDE database (PXD004807).

Martin L.A., Ribas R., Simigdala N., Schuster E., Pancholi S., Tenev T., Gellert P., Buluwela L., Harrod A., Thornhill A., Nikitorowicz-Buniak J., Bhamra A., Turgeon M.O., Poulogiannis G., Gao Q., Martins V., Hills M., Garcia-Murillas I., Fribbens C., Patani N., Li Z., Sikora M.J., Turner N., Zwart W., Oesterreich S., Carroll J., Ali S, & Dowsett M. (2017). Discovery of naturally occurring ESR1 mutations in breast cancer cell lines modelling endocrine resistance. Nature Communications, 8, 1865.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!