GTPase activation was performed according to standard procedures as described elsewhere [25] . In brief, Cells were harvested in magnesium-containing lysis buffer and the lysates were sonicated for 5 seconds and centrifuged for 30 minutes at 18,000× g and 4°C the Rho/Rac/Cdc42 Assay Reagent Kit (Thermo Scienti c, USA) according to the manufacturer's instructions. The primary antibodies used were anti-RhoA (Cell Signaling Technology, #2117, diluted at 1:1000), and anti-Rac1 (Millipore, 05-389, clone 23A8, diluted at 1:200), anti-Cdc42 (Cell Signaling Technology, #2462, diluted at 1:1000).
Rho rac cdc42 assay reagent kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Rho/Rac/Cdc42 Assay Reagent Kit is a tool for the detection and quantification of Rho, Rac, and Cdc42 GTPase activity. The kit provides the necessary reagents for performing the assay, including active GTPase-binding domains and associated detection components.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rho rac cdc42 assay reagent kit
1
GTPase Activation Protocol
2
Rho GTPase Activation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GTPase activation was performed according to standard procedures as described elsewhere [25] . In brief, Cells were harvested in magnesium-containing lysis buffer and the lysates were sonicated for5 seconds and centrifuged for 30 minutes at 18,000× g and4°C the Rho/Rac/Cdc42 Assay Reagent Kit(Thermo Scienti c, USA) according to the manufacturer's instructions. The primary antibodies used were anti-RhoA (Cell Signaling Technology, #2117, diluted at 1:1000), and anti-Rac1 (Millipore, 05-389, clone 23A8, diluted at 1:200), anti-Cdc42 (Cell Signaling Technology, #2462, diluted at 1:1000).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!