Images were acquired using a modified Olympus IX81 inverted epifluorescence microscope with an oil-immersion objective (UApo N 100x/1.49 NA, Olympus, Vienna, Austria). The sample was positioned with nanometer precision on a XYZ piezo stage (P-733.3DD, Physical Instruments) on top of a mechanical stage with a range of 1 × 1 cm adjusted by precision screws (TAO, JPK Instruments, Berlin, Germany). A tube-lens with an additional magnification of 1.6 was used to achieve a final imaging magnification of 160 (corresponding to a pixel size of 100 nm). Platelets were illuminated with a 642 nm laser light from a diode laser (Omicron-laserage Laserprodukte GmbH, Phoxx 642, Rodgau-Dudenhofen, Germany), a 488 nm laser light from a solid-state laser (diode-pumped, Toptica Photonics, Graefelfing, Germany), and a 405 nm laser light from a diode laser (Insaneware, Gladbeck, Germany). The signal was detected using an Andor iXonEM+ 897 (back-illuminated) EMCCD camera (16 μm pixel size). The following filter sets were used: dichroic filter (ZT405/488/561/640rpc, Chroma, Olching, Germany), emission filter (446/523/600/677 nm BrightLine quad-band band-pass filter, Semrock, Rochester, NY, USA), and an additional emission filter (HQ 700/75 M, NC209774, Chroma Technology GmbH, Olching, Germany).
Phoxx 642
Manufactured by Toptica
Sourced in Germany
The Phoxx 642 is a continuous-wave diode laser that produces highly stable and coherent light at a wavelength of 642 nanometers. It is designed for use in a variety of scientific and industrial applications requiring a reliable and precise light source.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using phoxx 642
1
Dual-color Imaging of Platelet Ultrastructure
2
High-resolution 3D Fluorescence Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Images were acquired using a modified Olympus
IX81 inverted epifluorescent
microscope with an oil-immersion objective (PlanApo N, 60×, NA
1.42, Olympus, Vienna, Austria). Samples were mounted on a XYZ piezo
stage (PI Mars; P-562, Physical Instruments) which has nanometer accuracy,
combined with a coarse mechanical stage with a travel range of 1 cm
× 1 cm (Hybrid, JPK Instruments, Berlin, Germany). A tube lens
with an additional magnification of 1.6 was used to achieve a final
imaging magnification of 96 (corresponding to a pixel size of 167
nm). ECs were illuminated with a 642 nm laser diode (Omicron-laserage
Laserprodukte GmbH, Phoxx 642, Rodgau-Dudenhofen, Germany) and a 488
nm laser (Toptica Photonics, Germany). Signals were collected using
an Andor iXonEM+ 897 (back-illuminated) EMCCD camera (16 μm
pixel size). The following filter sets were used: dichroic filter
(ZT405/488/561/640rpc, Chroma, Germany), emission filter (446/523/600/677
nm BrightLine quad-band band-pass filter, Semrock, Rochester, NY,
USA), and additional emission filters: ET 700/75 M, Chroma Technology
GmbH, Olching, Germany; ET 525/50 M, Chroma Technology GmbH, Olching,
Germany. For 3D measurements, a cylindrical lens (f = 500 mm; Thorlabs, Newton, USA) was placed into the detection path
of the microscope.
IX81 inverted epifluorescent
microscope with an oil-immersion objective (PlanApo N, 60×, NA
1.42, Olympus, Vienna, Austria). Samples were mounted on a XYZ piezo
stage (PI Mars; P-562, Physical Instruments) which has nanometer accuracy,
combined with a coarse mechanical stage with a travel range of 1 cm
× 1 cm (Hybrid, JPK Instruments, Berlin, Germany). A tube lens
with an additional magnification of 1.6 was used to achieve a final
imaging magnification of 96 (corresponding to a pixel size of 167
nm). ECs were illuminated with a 642 nm laser diode (Omicron-laserage
Laserprodukte GmbH, Phoxx 642, Rodgau-Dudenhofen, Germany) and a 488
nm laser (Toptica Photonics, Germany). Signals were collected using
an Andor iXonEM+ 897 (back-illuminated) EMCCD camera (16 μm
pixel size). The following filter sets were used: dichroic filter
(ZT405/488/561/640rpc, Chroma, Germany), emission filter (446/523/600/677
nm BrightLine quad-band band-pass filter, Semrock, Rochester, NY,
USA), and additional emission filters: ET 700/75 M, Chroma Technology
GmbH, Olching, Germany; ET 525/50 M, Chroma Technology GmbH, Olching,
Germany. For 3D measurements, a cylindrical lens (f = 500 mm; Thorlabs, Newton, USA) was placed into the detection path
of the microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!