Total protein (50 μg) was electrophoresed on a polyacrylamide gel with SDS and transferred onto a nitrocellulose membrane. Treated and untreated cells were incubated with anti-cyclin B1 1:200, cdc2 p34 1:200, and anti-MSH2 1:250 (Santa-Cruz Biotechnology, CA, USA) and Vinculin 1:10,000 (Sigma-Aldrich, St. Louis, MO, USA). Band analysis was performed with the Image J (Image Processing and Analysis in Java) software program.
Anti msh2
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-MSH2 is a primary antibody that recognizes the MSH2 protein. MSH2 is a key component of the DNA mismatch repair system, which is responsible for identifying and correcting errors that occur during DNA replication. The Anti-MSH2 antibody can be used to detect the presence and expression of MSH2 in various experimental applications.
Automatically generated - may contain errors
2 protocols using anti msh2
1
Protein Expression Analysis by Western Blot
2
Immunostaining of Allografts and Biopsies
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunohistochemical and immunofluorescent staining of allografts and colon biopsies were performed as previously described.21 (link) Cytokeratins, Ly6A/E and Dclk1 were stained using mouse anti-pancytokeratin monoclonal antibody (Novus Biologicals), rat anti-Ly6A/E (BD BioSciences) and rabbit polyclonal antibody to Dclk1 (Abcam). Rabbit anti-nitric oxide synthase 2 (Enzo Life Sciences), anti-arginase 1 (Sigma), and anti-MSH2 (Santa Cruz Biotechnology) polyclonal antibodies were used for Western blots.
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