Ammonium hydroxide nh3 h2o

Manufactured by Merck Group

Sourced in United States

Ammonium hydroxide (NH3·H2O) is a colorless, alkaline solution composed of ammonia dissolved in water. It is commonly used as a laboratory reagent and has applications in various chemical processes.

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3 protocols using ammonium hydroxide nh3 h2o

Liquid Chromatography-Mass Spectrometry Reagents

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Liquid chromatography‐mass spectrometry (LC‐MS) grade water, HPLC grade methanol (MeOH), acetonitrile (ACN), and ammonium acetate were purchased from Honeywell (Muskegon, MI, USA). Ammonium hydroxide (NH₃·H₂O) was purchased from Sigma‐Aldrich (St. Louis, MO, USA).

Zhang J., Cao J., Geng A., Wang H., Chu Q., Yan Z., Zhang X., Zhang Y, & Liu H. (2021). UHPLC‐QTOF/MS‐based comparative metabolomics in pectoralis major of fast‐ and slow‐growing chickens at market ages. Food Science & Nutrition, 10(2), 487-498.

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Synthesis and Characterization of Compounds

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RDV (purity ≥ 98 %), RMP (purity of 93 %), and RTP (purity of 98 %) were synthesized as previously reported [6 (link),18 (link)]. The structure and purity of each compound were identified by NMR and HPLC-UV. RN (purity of 98 %) was purchased from Target Molecule Corp. (Boston, MA, USA). Adenosine triphosphate (ATP, purity of 99 %), uridine triphosphate (UTP, purity of 96 %), ¹³C,¹⁵N-ATP (purity of 98 %), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB, purity ≥ 98 %), bis (p-nitrophenyl) phosphate (BNPP, purity ≥ 99 %), tris-(2-carboxyethyl)phosphine hydrochloride (TCEP, purity ≥ 98 %), Boc-Lys(Boc)-OH, ammonium hydroxide (NH₃∙H₂O, 28 % NH₃ in H₂O), and ammonium acetate (NH₄Ac) were obtained from Sigma-Aldrich (St. Louis, MO, USA). PhosSTOP™ EASYpack (PhosSTOP) was purchased from Roche (Basel, Switzerland). The Invitrosol™ LC/MS Protein Solubilizer Kit was purchased from ThermoFisher (MA, USA). Phosphoric acid (H₃PO₄, purity ≥ 85 %) and silver nitrate (AgNO₃) were obtained from Sinopharm Chemical Regent Co., Ltd. (Shanghai, China). Acetonitrile (ACN), formic acid (FA), and methanol (MeOH) were obtained from Merck (NJ, USA). Ultrapure water was obtained using a Millipore Milli-Q® Advantage A10 Purification System (Millipore, Molsheim, France). All other regents were of the highest quality commercially available.

Hu W., Chang L., Ke C., Xie Y., Shen J., Tan B, & Liu J. (2020). Challenges and stepwise fit-for-purpose optimization for bioanalyses of remdesivir metabolites nucleotide monophosphate and triphosphate in mouse tissues using LC–MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 194, 113806.

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Multifunctional Nanoparticle Synthesis and Characterization

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Tetraethyl orthosilicate (TEOS), dopamine hydrochloride, anhydrous ethanol, ammonium hydroxide (NH₃·H₂O), N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), and triphenylphosphine (TPP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Potassium permanganate (KMnO₄, 99%), and sodium carbonate (Na₂CO₃), were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). 2,2’-azobis[2-(2-imidazolin-2-yl) propane] dihydrochloride (AIBI) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Primary antibodies against Bcl-2 (Cat No. GTX100064), Bax (Cat No. GTX109683), Cytochrome c (Cyt-c, Cat No. GTX108585), cleaved-Caspase-9 (Cat No. GTX22324), cleaved-Caspase-3 (Cat No. GTX22302) and GAPDH (Cat No. GTX100118) were purchased from GeneTex (Irvine, CA, USA). All other chemicals and reagents were of the highest quality commercially available and used as received.

Hu H., Deng X., Song Q., Yang W., Zhang Y., Liu W., Wang S., Liang Z., Xing X., Zhu J., Zhang J., Shao Z., Wang B, & Zhang Y. (2021). Mitochondria-targeted accumulation of oxygen-irrelevant free radicals for enhanced synergistic low-temperature photothermal and thermodynamic therapy. Journal of Nanobiotechnology, 19, 390.

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