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Anti cd8 alpha antibody

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The Anti-CD8 alpha antibody is a protein that binds to the CD8 alpha chain, a cell surface glycoprotein expressed on the surface of cytotoxic T cells and a subset of natural killer cells. It is commonly used in flow cytometry and immunohistochemistry applications to identify and quantify CD8-positive cells.

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Lab products found in correlation

Vectastain elite abc kit, by Vector Laboratories (1 mentions) A1r ultra fast spectral scanning confocal microscope, by Nikon (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Goat anti rabbit igg h l alexa fluor 488, by Abcam (1 mentions) Anti ncam1 antibody, by Abcam (1 mentions) Benchmark ultra system, by Roche (1 mentions) Bx43 light microscope, by Olympus (1 mentions) Anti ki67 antibody, by Proteintech (1 mentions) Anti cd163 antibody, by Proteintech (1 mentions) Anti vegfa antibody, by Proteintech (1 mentions) Moticeasyscan, by Motic (1 mentions) Anti e cadherin antibody, by Proteintech (1 mentions) Anti n cadherin antibody, by Proteintech (1 mentions) Anti vimentin antibody, by Proteintech (1 mentions)

4 protocols using anti cd8 alpha antibody

1

CD8 Immunohistochemistry in Tumor Tissues

Cited 1 time
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Immunohistochemistry (IHC) was performed as previously described [31 (link)]. Briefly, the slides were de-paraffinised and activated with Tris-EDTA solution. The slides were incubated overnight at 4 °C and stained using the VECTASTAIN Elite ABC Kit (Vector Laboratories, CA, USA) and 3,3’-diaminobenzidine. The tumour tissues were stained with an anti-CD8 alpha antibody (clone EPR20305, Abcam, Tokyo, JPN).
Yamamoto S., Kato M., Takeyama Y., Azuma Y., Yukimatsu N., Hirayama Y., Otoshi T., Yamasaki T., Fujioka M., Gi M., Wanibuchi H, & Uchida J. (2023). Irradiation plus myeloid-derived suppressor cell-targeted therapy for overcoming treatment resistance in immunologically cold urothelial carcinoma. British Journal of Cancer, 128(12), 2197-2205.
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2

Quantifying Apoptosis and CD8+ T Cells in Tumor Samples

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Tumor sections (5 um) were fixed with 4% paraformaldehyde, blocked with 3% bovine serum albumin, and permeabilized with 0.1% Triton X-100 in PBS. The tumor sections were then incubated with anti-CD8 alpha antibody (1:200, Abcam, cat. no. ab217344) at 4 °C overnight and goat anti-rabbit IgG H&L (Alexa Fluor 488) (1:1000, Abcam, cat. no. ab150077) secondary antibody at room temperature for 1 h. Apoptotic tumor cells were stained using an in situ cell death detection kit (Roche) according to the manufacturer’s instructions. Images were acquired on a Nikon A1R Ultra-Fast Spectral Scanning Confocal Microscope.
Lu X., Miao L., Gao W., Chen Z., McHugh K.J., Sun Y., Tochka Z., Tomasic S., Sadtler K., Hyacinthe A., Huang Y., Graf T., Hu Q., Sarmadi M., Langer R., Anderson D.G, & Jaklenec A. (2020). Engineered PLGA microparticles for long-term, pulsatile release of STING agonist for cancer immunotherapy. Science translational medicine, 12(556), eaaz6606.
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3

Immunohistochemical Analysis of CD8+ T Cells and NK Cells

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The sections from the FFPE tissues of the cases were stained with CD8 antibody (Anti-CD8 alpha antibody [EPR20305], ready-to-use, Abcam) for CD8+ T-cell detection, or with NCAM1 (Anti-NCAM1 antibody [EPR21827], ready-to-use, Abcam) antibody for NK cell detection using a Ventana Benchmark Ultra system according to the manufacturer’s instructions. CD8 or NCAM1 positivity and hematoxylin–eosin staining patterns were evaluated, and then photographed with an Olympus BX43 light microscope by an expert pathologist.
Esmeray Sönmez E., Hatipoğlu T., Kurşun D., Hu X., Akman B., Yuan H., Erşen Danyeli A., Alacacıoğlu İ., Özkal S., Olgun A., Erdağ T.K., You H, & Küçük C. (2022). Whole Transcriptome Sequencing Reveals Cancer-Related, Prognostically Significant Transcripts and Tumor-Infiltrating Immunocytes in Mantle Cell Lymphoma. Cells, 11(21), 3394.
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4

Immunohistochemical Analysis of Cancer Biomarkers

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Thirty-six representative samples from C1 (N = 18) and C2 (N = 18) were selected for tissue microarray (TMA) construction, the TMA is prepared as previously described43 . For immunohistochemistry staining, the sections were stained using anti-Vimentin antibody (Proteintech, 1:2000), anti-Ki67 antibody (Proteintech, 1:5000), anti-Cyclin D antibody (Proteintech, 1:1500), anti- E-cadherin antibody (Proteintech, 1:2000), anti- N-cadherin antibody (Proteintech, 1:200), anti-ADH1A antibody (Abcam, 1:500), anti-CYP3A4 antibody (Proteintech, 1:200), anti-CD34 antibody (Proteintech, 1:1000), anti-VEGFA antibody (Proteintech, 1:200), anti-CD8-alpha antibody (Abcam, 1:500), anti-CD163 antibody (Proteintech, 1:1000). Images were captured by MoticEasyScan (Motic).
Fan Z., Zou X., Wang G., Liu Y., Jiang Y., Wang H., Zhang P., Wei F., Du X., Wang M., Sun X., Ji B., Hu X., Chen L., Zhou P., Wang D., Bai J., Xiao X., Zuo L., Xia X., Yi X, & Lv G. (2024). A transcriptome based molecular classification scheme for cholangiocarcinoma and subtype-derived prognostic biomarker. Nature Communications, 15, 484.
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