The receptor internalization assay was performed as described previously47 (link) with the following modifications: Cortical cultures were infected at 8 DIV with lentivirus for CPG2 KD (pFUGW-Cpg2-shRNA) and at 10 DIV with lentivirus for GFP-hCPG2 (pFUGW-GFP-hCPG2) reference protein or mutated variant molecular replacement. Western blots were probed with rabbit anti-GluA2 (1:1000; Abcam), mouse anti-GluN1 (1:2000; Temicula) or mouse anti-TfR (1:1000; Invitrogen) primary antibodies and developed using the Odyssey infrared system (LI-COR). Receptor internalization was quantified as described47 (link) and groups were compared using one-way ANOVA and Tukey’s post hoc test for multiple comparisons.
Mouse anti tfr
Manufactured by Thermo Fisher Scientific
The Mouse anti-TfR is a laboratory instrument used for the detection and quantification of transferrin receptor (TfR) protein in biological samples. It functions by binding to the TfR protein, allowing for its identification and measurement. The core purpose of this product is to facilitate the analysis of TfR expression in research and diagnostic applications.
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2 protocols using mouse anti tfr
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Receptor Internalization Assay with CPG2 Modulation
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Receptor Internalization Assay with CPG2 Modulation
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The receptor internalization assay was performed as described previously47 (link) with the following modifications: Cortical cultures were infected at 8 DIV with lentivirus for CPG2 KD (pFUGW-Cpg2-shRNA) and at 10 DIV with lentivirus for GFP-hCPG2 (pFUGW-GFP-hCPG2) reference protein or mutated variant molecular replacement. Western blots were probed with rabbit anti-GluA2 (1:1000; Abcam), mouse anti-GluN1 (1:2000; Temicula) or mouse anti-TfR (1:1000; Invitrogen) primary antibodies and developed using the Odyssey infrared system (LI-COR). Receptor internalization was quantified as described47 (link) and groups were compared using one-way ANOVA and Tukey’s post hoc test for multiple comparisons.
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