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Pseudokinase mlkl phospho s345

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Pseudokinase (MLKL) phospho-S345 is a lab equipment product that measures the phosphorylation of the pseudokinase MLKL (mixed lineage kinase domain-like protein) at serine residue 345. This phosphorylation event is a key step in the necroptosis signaling pathway.

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Lab products found in correlation

Anti poly adp ribose polymerase parp, by Cell Signaling Technology (2 mentions) Anti atp5a, by Abcam (2 mentions) Restore plus western blot stripping buffer, by Thermo Fisher Scientific (2 mentions) Anti hsp70, by BD (2 mentions) Nitrocellulose membrane, by Bio-Rad (2 mentions) Anti gapdh, by Abcam (2 mentions)

2 protocols using pseudokinase mlkl phospho s345

1

Western Blot Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein from each sample (10–20 μg) was separated by SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). Abs used for protein detection were the following: anti–poly(ADP-ribose) polymerase (PARP) (product no. 9542S; Cell Signaling Technology, Danvers, MA), anti–mixed lineage kinase domain-like pseudokinase (MLKL) phospho-S345 (product no. ab196436; Abcam), anti-GAPDH (product no. ab181602; Abcam), anti-HSP70 (product no. 610607; BD Biosciences), and anti-ATP5a (product no. ab176569; Abcam). Western blot membranes were stripped of Abs using Restore Plus Western Blot Stripping Buffer (Thermo Fisher Scientific) when additional Ab probing was required.
Zhu M., Barbas A.S., Lin L., Scheuermann U., Bishawi M, & Brennan T.V. (2018). Mitochondria Released by Apoptotic Cell Death Initiate Innate Immune Responses. ImmunoHorizons, 2(11), 384-397.
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2

Western Blot Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein from each sample (10–20 μg) was separated by SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). Abs used for protein detection were the following: anti–poly(ADP-ribose) polymerase (PARP) (product no. 9542S; Cell Signaling Technology, Danvers, MA), anti–mixed lineage kinase domain-like pseudokinase (MLKL) phospho-S345 (product no. ab196436; Abcam), anti-GAPDH (product no. ab181602; Abcam), anti-HSP70 (product no. 610607; BD Biosciences), and anti-ATP5a (product no. ab176569; Abcam). Western blot membranes were stripped of Abs using Restore Plus Western Blot Stripping Buffer (Thermo Fisher Scientific) when additional Ab probing was required.
Zhu M., Barbas A.S., Lin L., Scheuermann U., Bishawi M, & Brennan T.V. (2018). Mitochondria Released by Apoptotic Cell Death Initiate Innate Immune Responses. ImmunoHorizons, 2(11), 384-397.
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